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| Product Name | Glycolysis Assay Reagent |
| Catalog No. | CMTR-HMM-0005 |
| Description | Glycolysis is the pathway by which glucose is converted into pyruvate. The free energy released during this process is used to form high-energy compounds such as adenosine triphosphate (ATP) and reduced nicotinamide adenine dinucleotide (NADH). |
| Application | This product is used to analyze extracellular acidification (ECA/ECAR). Since lactate production is the primary contributor to extracellular acidification, this product provides a convenient and informative method for measuring cellular glycolytic flux. Such measurements provide insights into the central role of altered glycolytic activity in various physiological and pathophysiological processes, including cellular adaptation to hypoxia and ischemia, as well as the onset and progression of tumorigenesis. |
| Principle | The performance of the glycolysis assay enables sensitive and stable microplate measurements, overcoming many issues associated with the more cumbersome potentiometric pH method. The rate of extracellular acidification is calculated from the change in fluorescence signal over time, and since the measurement method is non-destructive and fully reversible (no consumption of pH-sensitive reagents), it can measure time courses and multiple drug treatments. |
| Assay Type | Quantitative |
| Detection Method | Fluorescent |
| Applicable Instruments | Microplate reader |
| Detection Time | 1 h 30 min |
| Sample Type | Adherent cells, Suspension cells |
| Storage | 4°C |
For research use only, not for clinical use.
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