| Product Name | Glutathione S-Transferase (GST) Activity Assay Reagent (Microplate Reader) |
| Catalog No. | ETR-HMM-0020 |
| Description | Glutathione S-transferase (GST) is a protein encoded by multiple genes with diverse cellular functions, widely distributed in various biological tissues such as animals, plants, and microorganisms. It participates in primary metabolism, secondary metabolism, detoxification, protecting plants from oxidative damage, and isolating foreign substances. Additionally, it functions as a ligand protein in plant hormone metabolism. |
| Application | Glutathione S-transferase catalyzes the reaction between reduced glutathione (GSH) and CDNB to form GS-DNB, which has a characteristic absorption peak at 340 nm. The activity of glutathione S-transferase can be characterized by changes in absorbance. |
| Applicable Instruments | Microplate Reader |
| Number of Testable Samples | 100 Samples |
| Matching | 96-well plate |
| Detection Time | 5 h (100 Samples) |
| Detection Method | CDNB method |
| Spectral Parameters | 340 nm |
| Signal Response | Incremental |
| Notes | All sample processing steps must be performed on ice to prevent inactivation. The crude enzyme solution must be measured on the same day after preparation; When measuring GST activity in cells, the cell count is recommended to be between 3 million and 5 million. When extracting GST from cells, add reagent 1 followed by grinding or ultrasonic treatment; do not use cell lysis buffer to treat cells. If the absorbance exceeds 1, it is recommended to dilute the crude enzyme solution with distilled water before measurement, and adjust the calculations accordingly; Maintain a constant temperature of 37°C (for mammals) or 25°C (for other species) during the reaction; It is recommended to add an appropriate amount of 37°C (mammalian) or 25°C (other species) distilled water to a beaker, place this beaker in a 37°C (mammalian) or 25°C (other species) constant-temperature water bath, and place the cuvette containing the reaction solution in this beaker during the reaction process; Accurately take readings at 10 seconds and 310 seconds to ensure the accuracy of the experimental results; If using a 96-well UV plate for measurement, use a multichannel pipette and perform measurements in batches to ensure consistent reaction times between groups; |
For research use only, not for clinical use.
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