| Product Name | Alkaline Phosphatase (ALP/AKP) Assay Reagent (Microplate Test) |
| Catalog No. | ETR-HMM-0031 |
| Description | Alkaline phosphatase decomposes sodium benzoate phosphate, producing free phenol and phosphate. The phenol reacts with 4-aminoantipyrine in an alkaline solution and is oxidized by potassium ferricyanide to form a red quinone derivative. The intensity of the red color can be used to determine the enzyme activity. |
| Application | This product uses the microplate method to measure ALP/AKP activity. |
| Applicable Instruments | Spectrophotometer; Microplate Reader; Semi-automatic Biochemical Analyzer |
| Number of Testable Samples | 96 Samples |
| Detection Time | 40 min (80 Samples) |
| Detection Method | Microplate test |
| Spectral Parameters | 520 nm |
| Standard Linear Range | 0.05-50.0 U/mL |
| Detection Limit | 0.5 U/mL |
| Inter-batch Variation CV % | 3.90 |
| Intra-batch Variation CV % | 1.2 |
| Coefficient of Variation CV % | 1.2 |
| Recovery Rate % | 99 |
| Storage | 2-8°C |
| Shelf Life | 3 months |
For research use only, not for clinical use.
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