| Product Name | Glutathione Reductase (GR) Activity Assay Reagent (UV Spectrophotometer) |
| Catalog No. | ETR-HMM-0013 |
| Description | Glutathione reductase (GR) is the primary flavoprotein enzyme responsible for maintaining the levels of reduced glutathione (GSH) in cells. As one of the key enzymes in the glutathione redox cycle, it catalyzes the reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH), helping to maintain the GSH/GSSG ratio in the body, It plays a significant role in oxidative stress responses and the ascorbic acid-glutathione cycle pathway. |
| Application | GR can catalyze the reduction of GSSG to GSH by NADPH, while NADPH is reduced to NADP+. NADPH has a characteristic absorption peak at 340 nm, and changes in absorbance can be used to characterize the activity of glutathione reductase. |
| Applicable Instruments | UV Spectrophotometer |
| Number of Testable Samples | 50 Samples |
| Matching | 1 mL quartz cuvette (d=10 mm) |
| Detection Time | 5 h (50 Samples) |
| Detection Method | NADPH method |
| Spectral Parameters | 340 nm |
| Signal Response | Decremental |
| Notes | The preparation of crude enzyme solutions and other processes must be conducted on ice. It is recommended to complete the assay on the same day after sample processing, and avoid repeated freezing and thawing of the crude enzyme solution. Reagent 1 contains a certain concentration of protein (approximately 0.1 mg/mL), so the protein content of the sample must be subtracted when measuring the protein concentration of the sample. During the reaction, maintain a constant temperature of 37°C (for mammals) or 25°C (for other species). It is recommended to add an appropriate amount of distilled water at 37°C (for mammals) or 25°C (for other species) to a beaker, place this beaker in a water bath maintained at 37°C (for mammals) or 25°C (for other species), and place the cuvette containing the reaction solution in this beaker during the reaction process; Accurately take readings at 10 seconds and 190 seconds to ensure the accuracy of the experimental results; |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2025 Alta DiagenoTech. All rights reserved.
