| Product Name | Glucose Assay Reagent |
| Catalog No. | CMTR-HMM-0001 |
| Description | Glucose is a ubiquitous energy source in most organisms, ranging from bacteria to humans. Carbohydrates are broken down into monosaccharides and disaccharides, with the majority being glucose. Through glycolysis and the tricarboxylic acid (TCA) cycle, glucose is oxidized, ultimately forming carbon dioxide and water, and generating the universal energy molecule ATP. Glucose is the primary energy source for the brain and a key component in protein synthesis and lipid metabolism. Therefore, measuring blood glucose levels is a critical diagnostic parameter for many metabolic disorders. |
| Application | This product is designed for the rapid and sensitive quantification of glucose levels in biological samples such as serum, plasma, other bodily fluids, food, and growth media. |
| Principle | Glucose oxidase mixes with glucose to make a product that reacts with a dye to produce color (λ = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The color and fluorescence produced are directly proportional to the glucose content. |
| Assay Type | Quantitative |
| Detection Method | Colorimetric; Fluorometric |
| Applicable Instruments | Microplate reader |
| Detection Time | 40 min |
| Sample Type | Cell culture supernatant, Other biological fluids, Cell lysate, Serum, Plasma, Tissue lysate, Urine |
| Detection Limit | 1 µM |
| Standard Linear Range | 1 µM - 10000 µM |
| Procedure | 1. Add the sample (deproteinized) and standard to the wells. 2. Add the reaction mixture and incubate at 37°C for 30 minutes. 3. Analyze using a microplate reader. |
| Storage | -20°C |
For research use only, not for clinical use.
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