Cat.No: HTK-HMM-0030 Datasheet
Specification Quantities
| Product Name | General Lysophosphatidic Acid (LPA) |
| Catalog No. | HTK-HMM-0030 |
| Description | Lysophosphatidic acid (LPA) is a biologically active phospholipid molecule with a variety of physiological and pathological effects. Through its G protein-coupled receptor, LPA is involved in a variety of biological effects, including cell proliferation, migration, cytokine secretion, and cytoskeletal rearrangement, thus playing an important role in the physiological and pathological processes of the organism. |
| Test Species | General |
| Synonyms | LysoPA; 1-Oleoyl-sn-Glycero-3-Phosphate; Lysophosphatidyl Acid |
| Applications | This kit is used for the detection of lysophosphatidic acid (LPA) and other multi-factors, after testing and other similar substances without significant cross-reactivity. No more than 8 indicators of multi-factors can be mixed in a single test. |
| Sample Type | Serum, plasma and other biological fluids. |
| Detection Methods | FLIA |
| Estimated Measurement Time | 1.5 h |
| Detection Range | 9.77-10000 ng/mL |
| Sensitivity | 3.257 ng/mL |
| Accuracy | Intra-lot variation: CV<10%; Inter-lot variation: CV<12%. |
| Detection Principle | The antibodies of the multi-factor detection kit (flow fluorescence luminescence method) such as lysopolyphosphatidic acid (LPA), are coated on magnetic beads to prepare solid-phase carriers. Standard substances or specimens, magnetic beads, and labeled VEGFA are added to the microwells, respectively. The labeled lysophosphatidic acid (LPA) and other multi-factor detection kits (flow cytometry fluorescence luminescence method) compete for binding with antibodies bound to solid-phase carriers. Then, after washing the unbound substances, add PE-labeled avidin, and thoroughly wash again before taking readings on the machine. The MFI value is negatively correlated with the multi-factor detection kits such as lysophosphatidic acid (LPA) in the samples (flow fluorescence luminescence method). |
| Procedures | 1. Standard, reagent, and sample preparation before experiment; 2. Add (standard, sample, magnetic beads, assay solution A) 50 μL of standard or sample and 10 μL of magnetic beads, add Detection Solution A 50 μL, incubate for 60 minutes at 37°C on a plate shaker; 3. Wash the plate with magnetic suction 3 times; 4. Add 100 μL of Detection Solution B and incubate at 37°C for 30 minutes with shaking; 5. Wash the plate magnetically 3 times; 6. Add 100 μL of Sheath Solution, vortex for 2 minutes and read. |
For research use only, not for clinical use.
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