Cat.No: HTK-HMM-0029 Datasheet
Specification Quantities
| Product Name | General Lysophosphatidic Acid (LPA) ELISA Kit |
| Catalog No. | HTK-HMM-0029 |
| Description | Lysophosphatidic acid (LPA) is a biologically active phospholipid molecule with a variety of physiological and pathological effects. Through its G protein-coupled receptor, LPA is involved in a variety of biological effects, including cell proliferation, migration, cytokine secretion, and cytoskeletal rearrangement, thus playing an important role in the physiological and pathological processes of the organism. |
| Test Species | General |
| Synonyms | LysoPA; 1-Oleoyl-sn-Glycero-3-Phosphate; Lysophosphatidyl Acid |
| Applications | This kit is used for the detection of lysophosphatidic acid (LPA), and there is no significant cross-reactivity with other similar substances. |
| Sample Type | Serum, plasma and other biological fluids. |
| Detection Methods | Competitive ELISA |
| Estimated Measurement Time | 2 h |
| Detection Range | 123.5-10,000 ng/mL |
| Sensitivity | 50.5 ng/mL |
| Accuracy | Intra-lot variation: CV<10%; Inter-lot variation: CV<12%. |
| Detection Principle | This kit uses competitive inhibition enzyme-linked immunosorbent assay to determine the level of the substance to be tested in the specimen. The microplate is coated with LPA monoclonal antibody to form a solid-phase carrier. Then, biotin-labeled LPA analogues and the antigen to be tested (standard or sample) are simultaneously added to the microplate Wells coated with the antibody. The antigen to be tested competes with the biotin-labeled LPA analogues for binding to the specific antibody. After incubation, the unbound substances are washed off. Then, HRP-labeled avidin is added. After incubation and thorough washing, the substrate TMB is added for color development. TMB is converted into blue under the catalysis of peroxidase and then into the final yellow under the action of acid. The higher the concentration of the specimen to be tested is, the more inhibited the binding of the labeled antigen and antibody will be, and the lighter the color will be. The depth of color development is positively correlated with the amount of enzyme, but negatively correlated with the content of the substance to be tested in the sample. The absorbance (O.D. value) is measured at a wavelength of 450nm using an microplate reader, and the sample concentration is calculated. |
| Procedures | 1. Prepare standards, reagents, and samples before the experiment; 2. Add 50 µL of sample (standard and sample), add 50 µL of Detection Solution A (prepared before use); incubate at 37°C for 1 hour. 3. Wash the plate 3 times; 4. Add 100 µL of Detection Solution B; incubate at 37°C for 30 min; 5. Wash the plate 5 times; 6. Add TMB Substrate 90 µL and incubate at 37°C for 10-20 minutes. 7. Add 50 µL of Termination Solution and read immediately at 450 nm. |
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