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| Product Name | FFPE Tissue Total RNA Extraction Kit (Column-Based, DNase Included) |
| Catalog No. | DREK-0023 |
| Description | A spin column-based kit specifically designed for the extraction of total RNA from formalin-fixed paraffin-embedded tissue sections. The optimized lysis buffer system removes paraffin and reverses formaldehyde-induced crosslinks, releasing RNA suitable for downstream RT-qPCR and gene expression analysis. The included on-column DNase I treatment removes genomic DNA contamination without a separate incubation step, and the inclusion of carrier RNA enhances recovery from samples with very low RNA content. |
| Intended Use | Purification of total RNA from FFPE tissue sections for gene expression analysis by RT-qPCR and microarray. |
| Principle / Technology | Silica membrane spin column purification with on-column DNase treatment and carrier RNA enhancement |
| Detection Method | RNA integrity and quantity assessed by spectrophotometry and Bioanalyzer; downstream RT-qPCR |
| Sample Type | Formalin-fixed paraffin-embedded tissue sections (5–20 µm thickness), microdissected FFPE samples |
| Performance Range / Specifications | RNA yield: 1–20 µg from 4 × 10 µm sections; typical A260/A280: 1.8–2.0; A260/A230: 1.8–2.2 |
| Sensitivity / LOD | RNA recovery from FFPE sections as small as 2 mm² surface area |
| Specificity | Purifies total RNA including mRNA, rRNA, miRNA; optimized lysis reverses formaldehyde crosslinks |
| Reaction Conditions / Protocol | Deparaffinize sections with xylene substitute; digest with proteinase K 15 min at 56 °C; heat treatment to reverse crosslinks; bind RNA to column; on-column DNase I treatment 15 min; wash; elute in 30–50 µL nuclease-free water |
| Components / Formulation | Lysis buffer, proteinase K, binding buffer, wash buffers, DNase I, DNase digestion buffer, carrier RNA, spin columns with collection tubes, nuclease-free water |
| Storage Conditions | Proteinase K and DNase I: –20 °C; other reagents: room temperature |
| Shelf Life | 12 months |
| Package Specifications | 50 preparations |
| Product Form | Liquid buffers; lyophilized enzymes; spin columns |
| Quality Control | RNA integrity verified by RT-qPCR amplification of 18S rRNA and at least one mRNA target; DNA removal confirmed by minus-RT control |
| Key Features | Carrier RNA additive improves recovery of picogram-level RNA from laser-capture microdissected samples |
| Purity | Extracted nucleic acid A260/A280: 1.8–2.0 (DNA), 1.9–2.1 (RNA); A260/A230 ≥1.8 |
| Concentration | Elution volume and yield as specified per sample type |
| Activity / Unit Definition | DNA/RNA binding capacity per column or per mg beads as specified |
| Molecular Weight | Genomic DNA >50 kb; RNA 0.1–10 kb range as applicable |
| Source / Origin | Silica membrane or magnetic bead technology; recombinant Proteinase K |
| pH Range / Optimal pH | Binding buffer pH 5.0–7.0 for silica-based binding |
| Shipping Conditions | Ambient temperature for most components; Proteinase K shipped with cold packs |
| Expiration Date / Stability | 12 months at recommended storage temperature |
| Regulatory / Compliance | Research use; IVD-grade versions available with full documentation |
| Compatibility | Compatible with manual spin columns, vacuum manifolds, and automated liquid handlers |
| Recommended Buffer System | Chaotropic salt binding buffer; ethanol-based wash; low-salt TE or water elution |
| Application Notes / Precautions | Pre-warm elution buffer to improve yield; avoid cross-contamination between samples; change pipette tips between steps |
| Batch-to-Batch Consistency | DNA yield and purity within ±15% of reference lot on standardized blood sample |
For research use only, not for clinical use.
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