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| Product Name | FFPE Genomic DNA Isolation Kit |
| Catalog No. | DREK-0094 |
| Description | A spin-column kit for isolating genomic DNA from formalin-fixed, paraffin-embedded tissue. A safer deparaffinization system and optimized lysis chemistry reduce the inhibitory effects of formalin crosslinking without requiring overnight digestion. |
| Intended Use | FFPE genomic DNA preparation for PCR, qPCR, library construction and next-generation sequencing. |
| Principle / Technology | Non-xylene deparaffinization, proteolytic digestion and selective silica-membrane purification. |
| Detection Method | Downstream genomic DNA analysis. |
| Sample Type | Formalin-fixed, paraffin-embedded tissue sections. |
| Performance Range / Specifications | Approximately 20-minute extraction after digestion; no overnight digestion required; high recovery and stable DNA quality; 50 reactions. |
| Specificity | Optimized deparaffinization and binding conditions enrich FFPE DNA while removing paraffin, protein and amplification inhibitors. |
| Reaction Conditions / Protocol | Deparaffinize the sample, digest the tissue, reverse or reduce crosslink-associated inhibition, bind DNA to the spin column, wash and elute. |
| Components / Formulation | Deparaffinization, lysis, binding, wash and elution reagents with protease, silica spin columns and collection tubes. |
| Storage Conditions | Store non-enzyme components at 15-25°C for up to 12 months; store at 2-8°C for longer periods. Follow the component label for protease storage. |
| Shelf Life | Non-enzyme components are stable for 12 months at 15-25°C under the stated conditions. |
| Package Specifications | 50 reactions. |
| Product Form | FFPE genomic DNA spin-column kit. |
| Quality Control | DNA recovery, purity and downstream PCR performance are evaluated with representative FFPE samples. |
| Key Features | Safer deparaffinization; no overnight digestion; approximately 20-minute post-digestion workflow; high-purity FFPE DNA. |
| Compatibility | PCR, qPCR, NGS library preparation and sequencing workflows. |
| Application Notes / Precautions | If stored solutions develop precipitates, equilibrate to room temperature and mix until fully dissolved; warming at 37°C may be used when needed. |
| Batch-to-Batch Consistency | FFPE DNA recovery and downstream amplification performance are controlled across batches. |
For research use only, not for clinical use.
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