FFPE Genomic DNA Isolation Kit
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FFPE Genomic DNA Isolation Kit

Cat.No: DREK-0094 Datasheet

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Product Name FFPE Genomic DNA Isolation Kit
Catalog No. DREK-0094
Description A spin-column kit for isolating genomic DNA from formalin-fixed, paraffin-embedded tissue. A safer deparaffinization system and optimized lysis chemistry reduce the inhibitory effects of formalin crosslinking without requiring overnight digestion.
Intended Use FFPE genomic DNA preparation for PCR, qPCR, library construction and next-generation sequencing.
Principle / Technology Non-xylene deparaffinization, proteolytic digestion and selective silica-membrane purification.
Detection Method Downstream genomic DNA analysis.
Sample Type Formalin-fixed, paraffin-embedded tissue sections.
Performance Range / Specifications Approximately 20-minute extraction after digestion; no overnight digestion required; high recovery and stable DNA quality; 50 reactions.
Specificity Optimized deparaffinization and binding conditions enrich FFPE DNA while removing paraffin, protein and amplification inhibitors.
Reaction Conditions / Protocol Deparaffinize the sample, digest the tissue, reverse or reduce crosslink-associated inhibition, bind DNA to the spin column, wash and elute.
Components / Formulation Deparaffinization, lysis, binding, wash and elution reagents with protease, silica spin columns and collection tubes.
Storage Conditions Store non-enzyme components at 15-25°C for up to 12 months; store at 2-8°C for longer periods. Follow the component label for protease storage.
Shelf Life Non-enzyme components are stable for 12 months at 15-25°C under the stated conditions.
Package Specifications 50 reactions.
Product Form FFPE genomic DNA spin-column kit.
Quality Control DNA recovery, purity and downstream PCR performance are evaluated with representative FFPE samples.
Key Features Safer deparaffinization; no overnight digestion; approximately 20-minute post-digestion workflow; high-purity FFPE DNA.
Compatibility PCR, qPCR, NGS library preparation and sequencing workflows.
Application Notes / Precautions If stored solutions develop precipitates, equilibrate to room temperature and mix until fully dissolved; warming at 37°C may be used when needed.
Batch-to-Batch Consistency FFPE DNA recovery and downstream amplification performance are controlled across batches.

For research use only, not for clinical use.

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