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| Product Name | Magnetic Bead-Based Soil DNA Extraction Kit |
| Catalog No. | DREK-0025 |
| Description | Specialized magnetic bead-based kit for extracting high-quality genomic DNA from soil, sediment, compost, and other environmental samples containing high levels of humic acids, polyphenols, and PCR inhibitors. A unique inhibitor removal (IRT) step using proprietary binding chemistry eliminates >99% of humic substances while magnetic bead technology captures DNA with high efficiency, producing DNA suitable for 16S rRNA sequencing, metagenomics, and functional gene qPCR. |
| Intended Use | Genomic DNA extraction from soil, sediment, compost, sludge, rhizosphere, and other environmental samples with high humic acid content; microbiome analysis; environmental metagenomics; soil biodiversity studies. |
| Principle / Technology | Chemical and mechanical lysis (bead beating + SDS/proteinase K); inhibitor removal technology (IRT) selectively precipitates humic acids while DNA remains soluble; magnetic beads bind DNA in optimized binding buffer; ethanol washes remove residual inhibitors; elution in low-salt buffer |
| Detection Method | Add 0.5 g soil to bead tube with lysis buffer; bead beat 10 min; add IRT reagent, incubate 5 min on ice, centrifuge; transfer supernatant; add binding buffer and magnetic beads; incubate 5 min; magnetic separate; wash 2×; elute in 50-100 µL |
| Sample Type | Soil (0.25-1 g), sediment, compost, sludge, rhizosphere soil; samples may be fresh, frozen, or dried |
| Sensitivity / LOD | Yields 2-15 µg DNA per 0.5 g soil depending on microbial biomass; DNA suitable for 16S qPCR without dilution; A260/A280 1.7-1.9 |
| Components / Formulation | Lysis buffer with SDS, bead beating tubes (0.1 mm and 0.5 mm zirconia beads), proteinase K (20 mg/mL), IRT inhibitor removal reagent, magnetic bead binding buffer, silica magnetic beads (50 mg/mL), wash buffer 1, wash buffer 2, elution buffer (Tris 10 mM, pH 8.0), collection tubes |
| Storage Conditions | Proteinase K at -20 °C; other components at 15-25 °C; magnetic beads do not freeze |
| Shelf Life | 12 months from manufacture date |
| Package Specifications | 50 preps, 100 preps, 250 preps |
| Product Form | Liquid reagents with bead beating tubes |
| Key Features | IRT technology removes >99% humic acids; combined bead beating + chemical lysis; magnetic bead format for automation; produces PCR-ready DNA without dilution; validated for 16S rRNA, ITS, and metagenomic sequencing; protocol ~60 min for 24 samples |
| Purity | DNA A260/A280 1.7-1.9; A260/A230 >1.5 (indicating low humic acid carryover); no detectable PCR inhibition at 1:10 dilution |
| Concentration | Elution volume and yield as specified per sample type |
| Activity / Unit Definition | DNA/RNA binding capacity per column or per mg beads as specified |
| Molecular Weight | Genomic DNA >50 kb; RNA 0.1–10 kb range as applicable |
| Source / Origin | Silica membrane or magnetic bead technology; recombinant Proteinase K |
| pH Range / Optimal pH | Binding buffer pH 5.0–7.0 for silica-based binding |
| Shipping Conditions | Ambient for most components; proteinase K cold pack; magnetic beads ambient |
| Expiration Date / Stability | 12 months; proteinase K 12 months at -20 °C after receipt; IRT reagent 24 months at RT |
| Regulatory / Compliance | For laboratory and research use only; RUO; ISO 9001 |
| Compatibility | Compatible with all soil types tested: clay, loam, sandy, peat, agricultural, forest, and contaminated soils; for highly alkaline or saline soils, increase IRT incubation to 10 min; extracted DNA compatible with 16S/ITS amplicon sequencing, shotgun metagenomics, and qPCR |
| Recommended Buffer System | Chaotropic salt binding buffer; ethanol-based wash; low-salt TE or water elution |
| Application Notes / Precautions | Pre-warm elution buffer to improve yield; avoid cross-contamination between samples; change pipette tips between steps |
| Batch-to-Batch Consistency | DNA yield and purity within ±15% of reference lot on standardized blood sample |
For research use only, not for clinical use.
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