Cell and Tissue Total RNA Isolation Kit with DNA Removal

Name: Cell and Tissue Total RNA Isolation Kit with DNA Removal
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Cat.No: DREK-0116 Datasheet

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Product Details Related Products

Product Name	Cell and Tissue Total RNA Isolation Kit with DNA Removal
Catalog No.	DREK-0116
Description	A comprehensive spin-column system for total RNA purification from animal cells and tissues. A genomic DNA adsorption filter and an on-column DNase digestion module reduce DNA contamination before RNA recovery.
Intended Use	Animal cell and tissue total RNA preparation for RT-PCR, real-time PCR and microarray analysis.
Principle / Technology	Chemical lysis, genomic DNA adsorption and optional on-column DNase digestion followed by selective silica-membrane RNA purification.
Detection Method	Downstream RNA analysis.
Sample Type	Animal tissues and cultured animal cells.
Performance Range / Specifications	50 reactions.
Reaction Conditions / Protocol	Lyse and homogenize the sample, pass the lysate through the DNA-removal filter, perform on-column DNase treatment where required, wash and elute total RNA.
Components / Formulation	Genomic DNA adsorption column, RNA purification column and on-column DNase digestion module.
Package Specifications	50 reactions.
Product Form	Spin-column total RNA isolation kit with DNA-removal module.
Key Features	Dual genomic DNA removal; no phenol/chloroform extraction; broad animal cell and tissue compatibility; straightforward room-temperature workflow.
Compatibility	RT-PCR, real-time PCR and microarray workflows.
Application Notes / Precautions	Do not overload the genomic DNA filter. Use the on-column DNase step when downstream assays are highly sensitive to residual DNA.

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For research use only, not for clinical use.