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| Product Name | Rapid Cell Total RNA Isolation Kit (5-Minute) |
| Catalog No. | DREK-0109 |
| Description | A rapid single-column kit for isolating total RNA from cultured animal cells. The alcohol-free lysis and binding workflow loads the lysate directly onto a silica membrane and removes protein, salts and other impurities through optimized washing. |
| Intended Use | Total RNA isolation for RT-PCR, qRT-PCR, Northern blotting, dot blotting, in vitro translation and high-throughput sequencing. |
| Principle / Technology | Direct silica-membrane binding after combined cell lysis and RNA binding, followed by rapid washing and elution. |
| Detection Method | Downstream RNA analysis. |
| Sample Type | Cultured animal cells, including a single well of a 96-well plate and inputs from approximately 1 × 10^4 cells. |
| Performance Range / Specifications | Approximately 5-minute room-temperature workflow; supports low-input cell samples; 100 reactions. |
| Reaction Conditions / Protocol | Lyse cells with the combined lysis/binding reagent, load the complete lysate directly onto the spin column without clarification, wash and elute purified RNA. |
| Components / Formulation | Silica RNA spin column with combined lysis/binding and wash reagents. |
| Storage Conditions | Store at 15-25°C. |
| Package Specifications | 100 reactions. |
| Product Form | Spin-column total RNA isolation kit. |
| Key Features | Five-minute workflow; no additional alcohol; no beta-mercaptoethanol, phenol or chloroform; low-input compatibility; high-purity RNA. |
| Shipping Conditions | Ship at room temperature. |
| Compatibility | RT-PCR, qRT-PCR, blotting, in vitro translation and sequencing workflows. |
| Application Notes / Precautions | Transfer the complete cell lysate to the column; centrifuging and discarding insoluble material before loading may reduce RNA yield. |
For research use only, not for clinical use.
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