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| Product Name | Cell and Tissue Genomic DNA Mini Extraction Kit |
| Catalog No. | DREK-0100 |
| Description | A silica-column kit for isolating genomic DNA from animal tissues, cultured cells and selected liquid samples without phenol/chloroform extraction or alcohol precipitation. |
| Intended Use | Genomic DNA preparation for restriction digestion, PCR, qPCR, viral DNA testing, library construction, Southern blotting and next-generation sequencing. |
| Principle / Technology | Proteolytic lysis followed by selective silica-membrane binding, washing and elution. |
| Detection Method | Downstream genomic DNA analysis. |
| Sample Type | 1-20 mg animal tissue, fewer than 5 × 10^6 cultured cells, saliva, milk and other compatible liquid samples. |
| Performance Range / Specifications | Approximately 30-60-minute extraction after sample digestion; column capacity approximately 35-40 µg DNA; 100 reactions. |
| Specificity | Selective genomic DNA recovery with removal of protein, RNA and other sample-derived inhibitors. |
| Reaction Conditions / Protocol | Digest and lyse the sample, establish binding conditions, load the lysate onto the spin column, wash and elute purified genomic DNA. |
| Components / Formulation | Cell/tissue lysis and digestion reagents, binding, wash and elution buffers, silica spin columns and collection tubes. |
| Storage Conditions | Store at 15-25°C. |
| Package Specifications | 100 reactions. |
| Product Form | Cell and tissue genomic DNA spin-column kit. |
| Quality Control | DNA yield, purity, integrity and downstream PCR performance are evaluated with representative tissue and cell samples. |
| Key Features | Broad cell and tissue compatibility; phenol-free purification; approximately 30-60-minute workflow; high-purity genomic DNA. |
| Shipping Conditions | Ship at room temperature. |
| Compatibility | Restriction digestion, PCR, qPCR, Southern blotting and sequencing workflows. |
| Application Notes / Precautions | Thorough digestion and homogenization are essential. Reduce input for nucleic-acid-rich tissues to prevent column blockage. |
| Batch-to-Batch Consistency | Genomic DNA yield and purity are controlled across production batches. |
For research use only, not for clinical use.
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