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| Product Name | Chloroform-Free Room-Temperature Total RNA Isolation Reagent |
| Catalog No. | DREK-0102 |
| Description | A low-toxicity total RNA isolation reagent for cultured cells, animal tissues and simple plant tissues. The room-temperature workflow precipitates protein, DNA and polysaccharide contaminants while retaining RNA in the upper solution. |
| Intended Use | Total RNA isolation for RT-PCR, qRT-PCR, Northern blotting, dot blotting, in vitro translation and high-throughput sequencing. |
| Principle / Technology | Chemical lysis and selective impurity precipitation without chloroform partitioning, followed by alcohol precipitation and washing of RNA. |
| Detection Method | Downstream RNA analysis. |
| Sample Type | Cultured cells, animal tissues and simple plant tissues. |
| Performance Range / Specifications | Total extraction time within approximately 1 hour; room-temperature processing; no chloroform; supplied for approximately 200 or 400 reactions. |
| Specificity | The chemistry separates RNA from precipitated protein, DNA, polysaccharides and other sample contaminants. |
| Reaction Conditions / Protocol | Homogenize the sample in the reagent, add the specified diluent, centrifuge to precipitate impurities, recover the RNA-containing upper phase, precipitate with alcohol, wash and dissolve the RNA. |
| Components / Formulation | Ready-to-use RNA lysis/isolation reagent with the associated diluent. |
| Package Specifications | 200 reactions; 400 reactions. |
| Product Form | Liquid total RNA isolation reagent. |
| Quality Control | RNA yield, purity and integrity are evaluated with representative cells and animal tissues. |
| Key Features | No chloroform; room-temperature workflow; low-toxicity handling; broad cell and tissue compatibility; recovers total RNA including small RNA species. |
| Compatibility | RT-PCR, qRT-PCR, blotting, in vitro translation and sequencing workflows. |
| Application Notes / Precautions | Use RNase-free consumables, ensure complete homogenization and carefully retain the RNA-containing upper solution after impurity precipitation. |
| Batch-to-Batch Consistency | RNA yield, purity and integrity are controlled across production batches. |
For research use only, not for clinical use.
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