| Product Name | Ethanol Assay Reagent |
| Catalog No. | CMTR-HMM-0048 |
| Description | Ethanol has an inhibitory effect on the central nervous system and, due to its psychoactive properties, is considered a drug. Ethanol is metabolized in the body as an energy-providing carbohydrate nutrient because it is metabolized into acetyl-CoA, which is an intermediate compound shared with glucose and can be used as an energy source or for biosynthesis in the citric acid cycle (TCA). In the human body, ethanol is converted by alcohol dehydrogenase into acetaldehyde, which is then converted by acetaldehyde dehydrogenase into acetic acid. Acetaldehyde is the product of the first step in alcohol metabolism and is associated with most of the toxic clinical effects of alcohol. |
| Application | This product can accurately quantify ethanol concentrations in various biological samples (e.g., serum, plasma, other body fluids, food, beverages, and growth media). |
| Principle | Ethanol oxidase oxidizes ethanol to produce H₂O₂, which reacts with the probe to generate color (maximum absorbance at 570 nm) and fluorescence (Ex/Em 535/587 nm). |
| Synonyms | Ethyl alcohol |
| Assay Type | Quantitative |
| Detection Method | Colorimetric; Fluorometric |
| Applicable Instruments | Microplate reader |
| Detection Time | 1 h |
| Sample Type | Cell culture supernatant, Drinks, Food samples, Other biological fluids, Serum, Plasma, Urine |
| Detection Limit | 10 nM |
| Standard Linear Range | 2 µM - 200 µM |
| Procedure | 1. Add the sample and standard to the wells. 2. Add the reaction mixture and incubate at 37°C for 30 minutes. 3. Analyze using a microplate reader. |
| Storage | -20°C |
For research use only, not for clinical use.
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