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| Product Name | Congo Red Amyloid Stain Kit, Alkaline Method |
| Catalog No. | ATRSA-0027 |
| Description | Congo red staining kit using the alkaline Puchtler method for demonstration of amyloid deposits in tissue sections. Amyloid appears as pink to red deposits under brightfield microscopy and exhibits characteristic apple-green birefringence under polarized light — the diagnostic gold standard for amyloid detection. Essential for amyloidosis diagnosis in kidney, heart, liver, and gastrointestinal biopsies. |
| Intended Use | Histological detection of amyloid deposits; systemic and localized amyloidosis diagnosis; classification of amyloid type (AA, AL, ATTR); renal amyloidosis evaluation; cardiac amyloidosis assessment; Alzheimer's disease brain tissue analysis. |
| Principle / Technology | Alkaline Congo red solution (80% ethanol saturated with NaCl and Congo red dye) selectively binds amyloid β-pleated sheet structure via hydrogen bonding; alkaline pH and high salt concentration maximize binding specificity; characteristic apple-green birefringence under polarized light confirms amyloid |
| Detection Method | Deparaffinize and hydrate; stain in alkaline Congo red 20-30 min; differentiate in alkaline alcohol (1% KOH in 80% ethanol); wash in water; counterstain with Mayer's hematoxylin 30 sec-2 min; blue in alkaline water; dehydrate, clear, mount |
| Sample Type | FFPE tissue sections (kidney, heart, liver, GI, brain, skin); 6-8 µm sections recommended for optimal birefringence; amyloid control slide strongly recommended |
| Sensitivity / LOD | Detects amyloid deposits of all types (AA, AL, ATTR, Aβ, Aβ2M); birefringence visible with deposits as small as 5-10 µm |
| Specificity | Alkaline method minimizes non-specific collagen staining; apple-green birefringence under polarized light is virtually pathognomonic for amyloid; false positives rare with proper differentiation |
| Reaction Conditions / Protocol | Staining: 20-30 min RT; differentiation: 5-15 sec (monitor microscopically); counterstain: 30 sec-2 min |
| Components / Formulation | Alkaline Congo red solution (Congo red in 80% ethanol saturated with NaCl, alkalized with NaOH), alkaline alcohol differentiation solution (1% KOH in 80% ethanol), Mayer's hematoxylin counterstain, positive control slide (renal or cardiac amyloidosis) |
| Storage Conditions | 15-25 °C; Congo red solution protected from light; alkaline alcohol kept tightly sealed |
| Shelf Life | 12 months from manufacture date |
| Package Specifications | Kit for 50 slides, 100 slides |
| Product Form | Liquid reagents, ready-to-use |
| Key Features | Puchtler alkaline method — highest amyloid specificity; diagnostic apple-green birefringence; ready-to-use alkaline Congo red (no daily preparation); Mayer's hematoxylin for nuclear detail; positive control slide included; compatible with polarized light microscopy |
| Purity | Congo red certified by Biological Stain Commission; ≥80% dye content; all chemicals histology grade |
| Concentration | As specified; ready-to-use or concentrate format |
| Activity / Unit Definition | Staining intensity and specificity validated on reference tissue/cell preparations |
| Molecular Weight | Varies by dye component as specified in product documentation |
| Source / Origin | Synthetic dyes and biological stains; certified by Biological Stain Commission where applicable |
| pH Range / Optimal pH | pH 6.0–7.0 for Romanowsky-type stains; varies by formulation |
| Shipping Conditions | Ambient; ethanol-containing solutions ship as flammable liquid (Class 3); KOH solution corrosive |
| Expiration Date / Stability | 12 months at 15-25 °C; alkaline Congo red stable when tightly sealed — ethanol evaporation changes staining characteristics; discard if heavy precipitate forms; Mayer's hematoxylin ripens with time — adjust staining time for older lots |
| Regulatory / Compliance | For laboratory and research use only; RUO; ethanol solutions flammable; KOH solution corrosive; ISO 9001 |
| Compatibility | Compatible with FFPE sections from all organs; 6-8 µm sections produce brightest birefringence; frozen sections can be stained but produce weaker birefringence; avoid acid-containing fixatives which may reduce Congo red binding; known amyloid-containing tissue (e.g., autopsy-proven cardiac amyloidosis) serves as optimal positive control |
| Recommended Buffer System | Use with provided buffer or phosphate buffer pH 6.8 as specified |
| Application Notes / Precautions | Avoid contact with skin and clothing; stains are permanent; use appropriate PPE |
| Batch-to-Batch Consistency | Staining intensity and differential contrast validated on reference specimens per lot |
For research use only, not for clinical use.
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