| Product Name | Citrate Assay Reagent |
| Catalog No. | CMTR-HMM-0049 |
| Description | Citric acid (HOOC-CH₂ -C(-OH)(-COOH)-CH₂ -COOH) is a key intermediate in the TCA cycle in mitochondria. It is formed by the addition of an acetyl group from acetyl-CoA, derived from the glycolytic pathway, to oxaloacetate. Citric acid can be transported out of the mitochondria and converted back to acetyl-CoA for fatty acid synthesis. |
| Application | This product is used to quantify citrate levels in biological samples. |
| Principle | Citric acid is converted to pyruvate via oxaloacetic acid. The quantification method for pyruvate involves converting the nearly colorless probe into a strongly colored (570 nm) and fluorescent (Ex/Em, 535/587 nm) product. |
| Synonyms | Citric acid |
| Assay Type | Quantitative |
| Detection Method | Colorimetric; Fluorometric |
| Applicable Instruments | Microplate reader |
| Detection Time | 40 min |
| Sample Type | Cell culture supernatant, Serum, Plasma, Urine, Tissue extracts |
| Detection Limit | 0.002 mM |
| Standard Linear Range | 0.002 mM - 10 mM |
| Procedure | 1. Add the sample and standard to the wells. 2. Add the reaction mixture and incubate at 37°C for 30 minutes. 3. Analyze using a microplate reader. |
| Storage | -20°C |
For research use only, not for clinical use.
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