Blood Calcium Content Assay Kit (Visible Spectrophotometers)
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Blood Calcium Content Assay Kit (Visible Spectrophotometers)

Cat.No: BBITK-HMM-0003 Datasheet

Specification Quantities

60T/50S:
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Product Details Related Products
Product Name Blood Calcium Content Assay Kit (Visible Spectrophotometers)
Catalog No. BBITK-HMM-0003
Description Blood calcium mainly refers to plasma calcium, which mostly exists in the form of ionic calcium and bound calcium. Among them, only ionic calcium directly plays a physiological role and is in a dynamic equilibrium state with bound calcium. It is mainly affected by blood pH. Blood calcium levels are related to many important physiological functions. Both too high and too low levels will affect normal physiological functions.
Testing Equipment Visible Spectrophotometers
Matching 1 mL glass cuvette (d=10 mm)
Number of Testable Samples 50 Samples
Estimated Measurement Time 1 h (50 Samples)
Storage Store at 4°C away from light
Self-contained Reagents CH3OH,MW=32.04,CAS: 67-56-1 CH3COCH3,MW=58.08,CAS: 67-64-1
Detection Principle Free calcium can react with GBHA in strong alkali solution to form a red complex (calcium-GBHA), the product has a characteristic absorption peak at 520 nm, and the change of absorbance value can be quantitatively detected by the level of blood calcium.
Detection Methods GBHA Method
Detection Wavelength 520 nm
Signal Response Incremental
Standard Calcium Chloride
Reference Standards y=1.0413x+0.0017 (R2=0.9995)
Standard Linear Range 0.05-1.0 µmol/mL
Detection Limit 0.05 µmol/mL
Note The blood should be collected in the morning on an empty stomach, and the measurement should be completed as soon as possible after blood collection; if A is greater than 1.0, it is recommended that the plasma to be measured be diluted with distilled water and then measured, and the calculation should be modified accordingly.
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