| Product Name | Angiotensin Converting Enzyme Inhibitor Activity Assay Kit (UV Spectrophotometer) |
| Catalog No. | BBITK-HMM-0033 |
| Description | Angiotensin-converting enzyme (ACE) is a zinc-containing dipeptide carboxyl peptidase, mainly existing in endothelial cells of various tissues such as the lungs, brain, and kidneys. Its main function is to inactivate bradykinin and catalyze the conversion of angiotensin I to angiotensin II. Angiotensin-converting enzyme inhibitors can reduce the production of angiotensin II and increase bradykinin activity. Thus, it becomes an ideal choice for treating diseases such as hypertension and heart failure. |
| Testing Equipment | UV Spectrophotometer |
| Matching | 1 mL glass cuvette (d=10 mm) |
| Number of Testable Samples | 48 Samples |
| Estimated Measurement Time | 4 h (48 Samples) |
| Self-contained Reagents | / |
| Detection Principle | Angiotensin-converting enzyme can catalyze the hydrolysis of the substrate N-[3- (2-furanyl) acryloyl] -L-phenylalanine-glycine-glycine (FAPGG) to generate furanyl-l-phenylalanine (FAP) and diglycetin (GG). Angiotensin-converting enzyme inhibitors can reduce the hydrolysis of FAPGG by inhibiting ACE activity. FAPGG has a characteristic absorption peak at 340 nm, and the activity of angiotensin-converting enzyme inhibitors can be characterized by the change in absorbance value. |
| Detection Methods | FAPGG Method |
| Detection Wavelength | 340 nm |
| Signal Response | Incremental |
| Note | Accurately complete the determination of absorbance values at the specified time points to ensure the accuracy and repeatability of the experimental results. If the A1 determination is greater than 1.2 or the ΔA determination is greater than 0.4, it is recommended to appropriately dilute the crude enzyme solution using reagent One before conducting the determination. If the determination of ΔA is less than 0.02, it is recommended to conduct the determination after appropriately increasing the enzymatic reaction time or the sample size, and make corresponding modifications during the calculation. To compare the inhibitory degrees of ACE by different reagents, extracts, drugs or tissues, the homogenates of reagents, extracts, drugs or tissues must be prepared at the same concentration and for the same reaction time for comparison. |
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