| Product Name | Blood Phosphorus Content Assay Kit (Microplate Reader) |
| Catalog No. | BBITK-HMM-0002 |
| Description | Blood phosphorus mainly refers to inorganic phosphorus existing in the blood in the form of inorganic phosphorus salts. The concentrations of calcium and phosphorus in plasma have a significant influence on the formation of bone salts and bone calcification, etc. The relative stability of their contents depends on the relative balance of the absorption and excretion of calcium and phosphorus, and the two metabolizations of calcification and decalcification. Blood phosphorus content can be used as one of the detection indicators for various diseases and has important applications in the field of medical detection. |
| Testing Equipment | Microplate Reader |
| Matching | 96-well plate |
| Number of Testable Samples | 100 Samples |
| Estimated Measurement Time | 4 h (100 Samples) |
| Storage | Store at 4°C away from light |
| Self-contained Reagents | H2SO4，MW=98.078，CAS: 7664-93-9 |
| Detection Principle | After the serum sample is processed to remove the organic phosphorus, the inorganic phosphorus can react with ammonium molybdate to generate phosphomolybdic acid, which is reduced by ferrous sulfate to generate blue compounds, and the product has a characteristic absorption peak at 660 nm, which can be quantitatively detected by the change of absorbance value of the blood phosphorus content. |
| Detection Methods | Molybdenum Blue Method |
| Detection Wavelength | 660 nm |
| Signal Response | Incremental |
| Standard | Phosphorus |
| Reference Standards | y=0.392x+0.0188 (R2=0.9991) |
| Standard Linear Range | 0.125-3.0 mmol/L |
| Detection Limit | 0.04 mmol/L |
| Note | The measurement should be completed as soon as possible after blood collection to avoid hemolysis; if the A-measurement is greater than 1.0, it is recommended that the samples to be measured be diluted with Reagent 1 appropriately before measurement; if the A-measurement is less than 0.05, it is recommended to change the ratio of serum and Reagent 1 in the extraction process appropriately before measurement, for example, 100 μL of serum added to 900 μL of Reagent 1, and the calculations will be modified accordingly. |
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