| Product Name | Serum Total Iron Binding Capacity (TIBC)Assay Kit (Visible Spectrophotometers) |
| Catalog No. | BBITK-HMM-0015 |
| Description | Serum total iron binding capacity (TIBC) refers to the ability of serum transferrin to bind all iron. Its content level is closely related to the occurrence and development of diseases such as iron deficiency anemia and acute hepatitis, and the level of transferrin can be indirectly reflected by measuring the total iron binding capacity. |
| Testing Equipment | Visible Spectrophotometers |
| Matching | 1 mL glass cuvette (d=10 mm) |
| Number of Testable Samples | 48 Samples |
| Estimated Measurement Time | 2 h (48 Samples) |
| Storage | Store at 4°C |
| Self-contained Reagents | / |
| Detection Principle | Serum transferrin can bind to Fe3+ under alkaline conditions. Unbound Fe3+ is reduced to Fe2+. Fe2+ can react with filozine to form a purple-red compound. The product has a characteristic absorption peak at 562 nm. At this time, the absorbance value at 562 nm is positively correlated with unbound Fe3+. After acidification treatment, Fe3+ bound to serum transferrin is released and further reduced to Fe2+. At this time, the absorbance is positively correlated with the total amount of Fe3+. Therefore, the total iron binding ability of serum can be characterized by the change in absorbance value. |
| Detection Methods | Phenanthrozine Colorimetric Method |
| Detection Wavelength | 562 nm |
| Signal Response | Incremental |
| Note | To ensure accurate results and avoid loss of reagents, please read the instruction manual carefully before the measurement, make sure the reagents are adequately stored and prepared, and the operation steps are clear, and be sure to take 2-3 samples with expected differences for pre-calibration. |
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