| Product Name | Blood Sodium Content Assay Kit (Microplate Reader) |
| Catalog No. | BBITK-HMM-0012 |
| Description | Blood sodium plays a significant role in maintaining normal extracellular fluid volume and osmotic pressure, as well as the acid-base balance of body fluids. Its content is related to a variety of important physiological functions. Both excessively high and low levels can affect normal physiological functions. |
| Testing Equipment | Microplate Reader |
| Matching | 96-well plate |
| Number of Testable Samples | 100 Samples |
| Estimated Measurement Time | 3 h (100 Samples) |
| Storage | Store at 4°C |
| Self-contained Reagents | C2H6O，MW=46.07，CAS: 64-17-5 |
| Detection Principle | Sodium ions in serum can generate precipitation with potassium pyroantimonate in weakly alkaline solution, and the resulting turbidity is proportional to the concentration of sodium ions within a certain range, and the serum sodium content can be quantitatively detected by determining its turbidity. |
| Detection Methods | Potassium Pyroantimonate Method |
| Detection Wavelength | 520 nm |
| Signal Response | Incremental |
| Standard | Sodium |
| Reference Standards | y=19.283x+0.0142 (R2=0.9995) |
| Standard Linear Range | 0.005-0.06 mol/L |
| Detection Limit | 0.003 mol/L |
| Note | After blood collection, the content determination should be completed as soon as possible, avoiding the use of sodium citrate anticoagulant; if the absorbance value exceeds the linear range of the standard absorbance value: higher than the highest value, it is recommended that the sample to be tested should be appropriately diluted with anhydrous ethanol and then measured; lower than the lowest value, it is recommended to appropriately adjust the ratio of serum to anhydrous ethanol and then measured, and the calculations should be modified accordingly. |
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