| Product Name | Blood Magnesium Content Assay Kit (Microplate Reader) |
| Catalog No. | BBITK-HMM-0010 |
| Description | Magnesium ions in the blood are important cations in the body. They can act as activators of various enzymes and cofactors of oxidative phosphorylation, playing a significant role in maintaining the relative stability of the internal environment of the body and normal life activities. Their content is related to multiple metabolic processes. Both excessively high and low levels can affect the normal physiological functions of the body. |
| Testing Equipment | Microplate Reader |
| Matching | 96-well plate |
| Number of Testable Samples | 100 Samples |
| Estimated Measurement Time | 2 h (100 Samples) |
| Storage | Store at 4°C away from light |
| Self-contained Reagents | / |
| Detection Principle | Magnesium ions are hydroxidized to colloidal particles in alkaline medium, and further combined with datan yellow to show orange-red color, the product has a characteristic absorption peak at 540 nm, and serum magnesium content can be quantitatively detected by the change of absorbance value. |
| Detection Methods | Dartan Yellow Colorimetric Method |
| Detection Wavelength | 540 nm |
| Signal Response | Incremental |
| Standard | Magnesium |
| Reference Standards | y=0.0614x+0.0493 (R2=0.9987) |
| Standard Linear Range | 1.0-6.0 µmol/mL |
| Detection Limit | 1.0 µmol/mL |
| Note | Blood samples should avoid hemolysis, serum separation should be completed as soon as possible after blood collection, and avoid the use of sodium citrate anticoagulant; serum samples should be completed as soon as possible to determine the content of serum samples, and in the experimental process as far as possible to avoid strong light exposure; if the A determination is greater than 1.0, it is recommended that the serum to be tested be appropriately diluted with distilled water before determination, and the calculation is modified accordingly. |
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