| Product Name | Blood Calcium Content Assay Kit (Microplate Reader) |
| Catalog No. | BBITK-HMM-0004 |
| Description | Blood calcium mainly refers to plasma calcium, which mostly exists in the form of ionic calcium and bound calcium. Among them, only ionic calcium directly plays a physiological role and is in a dynamic equilibrium state with bound calcium. It is mainly affected by blood pH. Blood calcium levels are related to many important physiological functions. Both too high and too low levels will affect normal physiological functions. |
| Testing Equipment | Microplate Reader |
| Matching | 96-well plate |
| Number of Testable Samples | 100 Samples |
| Estimated Measurement Time | 2 h (100 Samples) |
| Storage | Store at 4°C away from light |
| Self-contained Reagents | CH3OH，MW=32.04，CAS: 67-56-1 CH3COCH3，MW=58.08，CAS: 67-64-1 |
| Detection Principle | Free calcium can react with GBHA in strong alkali solution to form a red complex (calcium-GBHA), the product has a characteristic absorption peak at 520 nm, and the change of absorbance value can be quantitatively detected by blood calcium level. |
| Detection Methods | GBHA Method |
| Detection Wavelength | 520 nm |
| Signal Response | Incremental |
| Standard | Calcium Chloride |
| Reference Standards | y=0.4767x+0.0158 (R2=0.9995) |
| Standard Linear Range | 0.125-2.5 µmol/mL |
| Detection Limit | 0.125 µmol/mL |
| Note | The blood should be collected in the morning on an empty stomach, and the measurement should be completed as soon as possible after blood collection; if A is greater than 1.0, it is recommended that the plasma to be measured be diluted with distilled water and then measured, and the calculation should be modified accordingly. |
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