| Product Name | ATP Assay Reagent |
| Catalog No. | CMTR-HMM-0015 |
| Description | ATP is a triphosphate nucleoside that provides energy for many processes in living cells, such as muscle contraction, nerve impulse transmission, and chemical synthesis. |
| Application | This product is used to detect ATP as low as 1 μM in various samples. |
| Principle | Glycerol is phosphorylated in an ATP-dependent reaction catalyzed by glycerol kinase, yielding 3-phosphoglycerate. - Then, 3-phosphoglycerate is converted into glycerol phosphate and hydrogen peroxide by glycerol phosphate oxidase. - Hydrogen peroxide is then processed by peroxidase, reacting with the probe to produce red (λmax = 570 nm) and fluorescent (Ex/Em = 535/587 nm) signals. |
| Synonyms | Adenosine 5' triphosphate |
| Assay Type | Quantitative |
| Detection Method | Colorimetric; Fluorometric |
| Applicable Instruments | Microplate reader |
| Detection Time | 1 h |
| Sample Type | Other biological fluids, Cell lysate, Serum, Plasma, Urine, Tissue extracts |
| Detection Limit | 1 µM |
| Procedure | 1. Add the sample and standard to the wells. 2. Add the reaction mixture and incubate at 37°C for 30 minutes. 3. Analyze using a microplate reader. |
| Storage | -20°C |
For research use only, not for clinical use.
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