| Product Name | Aspartate Aminotransferase Activity Assay Reagent |
| Catalog No. | CMTR-HMM-0013 |
| Description | Aspartate aminotransferase (AST), also known as aspartate transaminase and glutamic-oxaloacetic transaminase (GOT), is a transaminase similar to alanine transaminase (ALT), which is more liver-specific. Although AST is typically used as part of diagnostic liver function testing in clinical settings, it has broader clinical applications because it may also be elevated in diseases affecting other organs, such as the heart or muscles in myocardial infarction, as well as acute pancreatitis, acute hemolytic anemia, severe burns, acute kidney disease, musculoskeletal disorders, and trauma. |
| Application | This product is used to determine AST activity. |
| Principle | The amino group on aspartic acid is transferred to α-ketoglutarate. The products of this reversible transamination reaction are oxaloacetate and glutamate. Glutamate detection is performed in a reaction that converts an almost colorless probe into a colored substance (λmax = 450 nm). |
| Synonyms | AATC_HUMAN; Aspartate aminotransferase; ASTQTL1; cAspAT; cCAT; GIG18; SGOT; GOT1 |
| Assay Type | Quantitative |
| Detection Method | Colorimetric |
| Applicable Instruments | Microplate reader |
| Detection Time | 1 h |
| Sample Type | Cell culture supernatant, Plasma, Saliva, Serum, Tissue extracts |
| Detection Limit | 10 mU/well |
| Procedure | 1. Add the sample and standard to the wells. 2. Add the reaction mixture. 3. Incubate at 37°C, analyze with a microplate reader after 10 minutes, and analyze again after 60 minutes. |
| Storage | -20°C |
For research use only, not for clinical use.
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