| Product Name | Cholesterol/ Cholesteryl Ester Assay Reagent |
| Catalog No. | CMTR-HMM-0029 |
| Description | Cholesterol is an essential molecule in all animal life. It is involved in normal development and disease. Elevated serum cholesterol levels are an indicator of diseases such as heart disease. |
| Application | This product is used for sensitive quantitative analysis of total cholesterol, free cholesterol, and cholesterol esters. |
| Principle | Cholesterol esterase hydrolyzes it into cholesterol. Cholesterol is then oxidized by cholesterol oxidase to produce hydrogen peroxide. Hydrogen peroxide is subsequently processed by the enzyme and reacts with the probe to produce red (λmax = 570 nm) and fluorescent (Ex/Em = 535/587 nm) products. |
| Assay Type | Quantitative |
| Detection Method | Colorimetric; Fluorometric |
| Applicable Instruments | Microplate reader |
| Detection Time | 1 h |
| Sample Type | Cell culture supernatant, Other biological fluids, Serum, Plasma, Urine, Tissue extracts |
| Procedure | 1. Add samples and standards to the wells. 2. Add the total cholesterol reaction mixture (containing esterase) or free cholesterol reaction mixture (without esterase) and incubate at 37°C for 60 minutes. 3. Analyze using a microplate reader. |
| Storage | -20°C |
For research use only, not for clinical use.
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