| Product Name | Triglyceride Assay Reagent |
| Catalog No. | CMTR-HMM-0006 |
| Description | Triglycerides are a major form of fat (lipid) in the blood. They are the primary means of storing excess energy; if energy intake exceeds expenditure, the excess energy is converted into triglycerides and stored in fat cells. |
| Application | This product is intended for the determination of triglyceride concentrations in mammalian samples. |
| Principle | Triglycerides are converted into free fatty acids and glycerol by lipase. Glycerol is then phosphorylated by glycerol kinase and subsequently oxidized by phosphoglycerate oxidase, producing hydrogen peroxide. Hydrogen peroxide reacts with the probe via peroxidase, producing color (spectrophotometry, λ = 570 nm) and fluorescence (Ex/Em = 535/587 nm). |
| Synonyms | TG; Triacylglyceride; triglycerides |
| Assay Type | Quantitative |
| Detection Method | Colorimetric; Fluorometric |
| Applicable Instruments | Microplate reader |
| Detection Time | 1 h 20 min |
| Sample Type | Cell lysate, Hep Plasma, Other biological fluids, Serum, Urine, Tissue extracts |
| Detection Limit | 2 µM |
| Procedure | 1. Add the sample and standard to the wells. 2. Add the analysis buffer and lipase, and incubate for 20 minutes. 3. Add the triglyceride reaction mixture and incubate for 60 minutes. 4. Analyze using a microplate reader. |
| Storage | -20°C |
For research use only, not for clinical use.
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