| Product Name | ALDH Activity Assay Reagent |
| Catalog No. | CMTR-HMM-0034 |
| Description | NAD-dependent aldehyde dehydrogenase (ALDH) plays a crucial role in cellular detoxification processes. It oxidizes various aldehydes and generates the corresponding carboxylic acids. ALDH is present in all cellular compartments. ALDH gene mutations (loss of function) can lead to human diseases such as type II hyperprolinemia, pyridoxine-dependent epilepsy, and hyperammonemia. Recent research has shown that elevated ALDH activity can lead to various malignant tumors, serve as a marker for cancer stem cells, and be associated with poor prognosis. |
| Application | This product is used for quantitative analysis of ALDH enzyme activity. |
| Principle | Acetaldehyde is oxidized by ALDH to produce NADH, which reduces the colorless probe to a colored product with strong absorbance at 450 nm. This assay can detect ALDH activity of < 0.5 mU in various samples. |
| Assay Type | Quantitative |
| Detection Method | Colorimetric |
| Applicable Instruments | Microplate reader |
| Detection Time | 30 min |
| Sample Type | Cell lysate, Serum, Plasma, Tissue Homogenate, Tissue lysate |
| Detection Limit | 0.1 mU/well |
| Procedure | 1. Add the sample and standard to the wells 2. Add the reaction mixture and incubate for 20–60 minutes 3. Analyze using a microplate reader at 5 minutes and 20–60 minutes, respectively |
| Storage | -20°C |
For research use only, not for clinical use.
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