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| Product Name | Alanine Transaminase Activity Assay Reagent |
| Catalog No. | CMTR-HMM-0012 |
| Description | ALT is a transaminase primarily concentrated in the cytoplasm of liver cells, catalyzing the transamination reaction between alanine and α-keto acids. Currently, ALT is considered a sensitive indicator of liver cell damage. |
| Application | This product is used to quantify alanine transaminase (ALT) activity in mammalian samples. |
| Principle | ALT transfers the amino group from alanine to α-ketoglutarate, producing pyruvate and glutamate. The detection of pyruvate is achieved through a reaction that converts an almost colorless probe into a colored (ODmax = 570 nm) and fluorescent (Ex/Em = 535/587 nm) form. |
| Synonyms | Alanine Aminotransferase; ALT |
| Assay Type | Quantitative |
| Detection Method | Colorimetric; Fluorometric |
| Applicable Instruments | Microplate reader |
| Detection Time | 1 h 20 min |
| Sample Type | Cell culture media, Other biological fluids, Cell lysate, Serum, Plasma, Urine, Tissue extracts |
| Detection Limit | 10 mU/well |
| Procedure | 1. Add samples and standards to the wells. 2. Add the reaction mixture and incubate at 37°C for 10 minutes. 3. Analyze every 2-3 minutes for 60 minutes using a microplate reader in kinetic mode at 37°C. |
| Storage | -20°C |
For research use only, not for clinical use.
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