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| Product Name | Zeatin |
| Catalog No. | RESK-0591 |
| Description | Zeatin is a natural plant cytokinin (cytokinins, CKs) present in higher plants. It was first discovered and isolated from young corn cobs, and was later also found in coconut milk together with its derivatives. As a cell growth regulator, zeatin promotes lateral bud growth, stimulates cell differentiation (apical dominance), promotes callus formation and seed germination, prevents leaf senescence, reverses toxin damage to buds, and suppresses excessive root formation. High concentrations of zeatin can also induce adventitious bud differentiation. Zeatin exists as cis and trans isomers. The trans isomer has strong biological activity, whereas the cis isomer is relatively weak. This product is a mixture of the two isomers with a purity of >98% and is suitable for plant tissue culture. |
| Intended Use | Used as a plant growth regulator (cytokinin) in plant tissue culture. |
| Principle / Technology | Zeatin is a naturally occurring cytokinin of higher plants. It exists as cis and trans isomers; the trans isomer is highly biologically active while the cis isomer is relatively weak. Functionally it promotes lateral bud growth, stimulates cell differentiation, promotes callus formation and seed germination, prevents leaf senescence, and inhibits excessive root formation. |
| Detection Method | Purity determined by HPLC. |
| Performance Range / Specifications | Synonym: 6-(4-Hydroxy-3-methyl-but-2-enylamino)purine CAS No.: 13114-27-7 Molecular formula: C10H13N5O Molecular weight: 219.24 Appearance: White to yellow powder Melting point: 207-208 °C Solubility: Soluble in 1 M acetic acid and 1 M NaOH Purity (HPLC): >98% |
| Reaction Conditions / Protocol | 1) Stock solution preparation: Upon receipt, centrifuge the vial at low speed. Fully dissolve the powder in 5 mL of 1 M NaOH (or 1 M acetic acid). Then add double-distilled water with stirring to prepare a stock solution of 1 mg/mL or higher, ensuring thorough mixing. Aliquot the stock solution and store frozen; avoid repeated freeze-thaw cycles. 2) Recommended application concentrations: a) Promotion of callus shoot induction (must be used in combination with an auxin): 1 mg/L; b) Promotion of fruit set: zeatin 100 mg/L + GA3 500 mg/L + NAA 20 mg/L, sprayed onto fruits 10, 25, and 40 days after flowering; c) Delaying leaf yellowing of vegetables: 20 mg/L spray. |
| Storage Conditions | Store at -25 to -15 °C. |
| Package Specifications | 10 mg, 100 mg |
| Product Form | White to yellow powder |
| Purity | >98% (HPLC) |
| Concentration | Stock solution: 1 mg/mL or higher; typical working concentrations range from 1 mg/L to 100 mg/L depending on the application. |
| Molecular Weight | 219.24 |
| Source / Origin | A natural cytokinin of higher plants, first discovered and isolated from young corn cobs; also found in coconut milk together with its derivatives. |
| Expiration Date / Stability | Aliquot the stock solution and store frozen; avoid repeated freeze-thaw cycles. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Used in combination with auxins for callus shoot induction; can be applied together with GA3 and NAA to promote fruit set. |
| Recommended Buffer System | Dissolve the powder in 1 M NaOH or 1 M acetic acid, then dilute with double-distilled water to the desired concentration. |
| Application Notes / Precautions | Centrifuge the vial at low speed upon receipt before opening. Aliquot stock solutions and avoid repeated freeze-thaw cycles. For callus shoot induction, zeatin must be used in combination with an auxin. |
For research use only, not for clinical use.
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