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| Product Name | RPMI 1640 Medium (with L-Glutamine; without Sodium Pyruvate, HEPES and Antibiotics) |
| Catalog No. | RESK-0632 |
| Description | RPMI 1640 Medium is named after the Roswell Park Memorial Institute where it was developed, and 1640 is the medium designation. It is a modification of McCoy's 5A medium and uses a bicarbonate buffer system. RPMI 1640 Medium was originally developed for the suspension or monolayer culture of human leukemic cells, and was later found to be suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes and cancer cells. It is especially suitable for the culture of suspension cells and is one of the most widely used media. RPMI 1640 Medium differs from other media in that it contains reduced glutathione and high concentrations of vitamins. It also contains biotin, vitamin B12 and para-aminobenzoic acid, which are absent from EMEM and DMEM, as well as high concentrations of choline chloride and inositol. This product contains: D-Glucose, Phenol Red, and L-Glutamine. This product does not contain: Sodium Pyruvate, HEPES, or antibiotics. |
| Intended Use | Cell culture medium suitable for the culture of a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes and cancer cells, and especially suitable for suspension cell culture. |
| Principle / Technology | Bicarbonate-buffered basal medium providing amino acids, vitamins, inorganic salts and glucose for mammalian cell growth; contains reduced glutathione and high concentrations of vitamins to support cell proliferation in vitro. |
| Sample Type | Mammalian cells, especially suspension cells (e.g., HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes and cancer cells); applicable species: human. |
| Performance Range / Specifications | Contains: D-Glucose (2000 mg/L), Phenol Red (5 mg/L), L-Glutamine (300 mg/L) Without: Sodium Pyruvate, HEPES and antibiotics Buffer system: bicarbonate buffer system |
| Reaction Conditions / Protocol | Use under standard mammalian cell culture conditions with a bicarbonate/CO2 buffering environment (supplement with serum and antibiotics as required by the specific cell type). |
| Components / Formulation | Amino Acids Glycine: 10.0 mg/L (0.133 mM) L-Arginine: 200.0 mg/L (1.149 mM) L-Asparagine: 50.0 mg/L (0.379 mM) L-Aspartic acid: 20.0 mg/L (0.150 mM) L-Cystine 2HCl: 65.0 mg/L (0.208 mM) L-Glutamic Acid: 20.0 mg/L (0.136 mM) L-Glutamine: 300.0 mg/L (2.055 mM) L-Histidine: 15.0 mg/L (0.097 mM) L-Hydroxyproline: 20.0 mg/L (0.153 mM) L-Isoleucine: 50.0 mg/L (0.382 mM) L-Leucine: 50.0 mg/L (0.382 mM) L-Lysine hydrochloride: 40.0 mg/L (0.219 mM) L-Methionine: 15.0 mg/L (0.101 mM) L-Phenylalanine: 15.0 mg/L (0.091 mM) L-Proline: 20.0 mg/L (0.174 mM) L-Serine: 30.0 mg/L (0.286 mM) L-Threonine: 20.0 mg/L (0.168 mM) L-Tryptophan: 5.0 mg/L (0.025 mM) L-Tyrosine disodium salt dihydrate: 29.0 mg/L (0.111 mM) L-Valine: 20.0 mg/L (0.171 mM) Vitamins Biotin: 0.2 mg/L (0.00082 mM) Choline chloride: 3.0 mg/L (0.021 mM) D-Calcium pantothenate: 0.25 mg/L (0.00052 mM) Folic Acid: 1.0 mg/L (0.002 mM) Niacinamide: 1.0 mg/L (0.008 mM) Para-Aminobenzoic Acid: 1.0 mg/L (0.007 mM) Pyridoxine hydrochloride: 1.0 mg/L (0.005 mM) Riboflavin: 0.2 mg/L (0.00053 mM) Thiamine hydrochloride: 1.0 mg/L (0.003 mM) Vitamin B12: 0.005 mg/L (0.0000037 mM) i-Inositol: 35.0 mg/L (0.194 mM) Inorganic Salts Calcium nitrate (Ca(NO3)2-4H2O): 100.0 mg/L (0.424 mM) Magnesium Sulfate (MgSO4, anhyd.): 48.84 mg/L (0.407 mM) Potassium Chloride (KCl): 400.0 mg/L (5.333 mM) Sodium Chloride (NaCl): 6000.0 mg/L (103.448 mM) Sodium Phosphate dibasic (Na2HPO4-7H2O): 800.0 mg/L (2.985 mM) Sodium Bicarbonate (NaHCO3): 2000.0 mg/L (23.8 mM) Other Components D-Glucose (Dextrose): 2000.0 mg/L (11.1 mM) Glutathione (reduced): 1.0 mg/L (0.003 mM) Phenol Red: 5.0 mg/L (0.013 mM) |
| Storage Conditions | Store at 2-8 °C, protected from light. |
| Shelf Life | 1 year. |
| Package Specifications | 500 mL. |
| Product Form | Liquid. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes and cancer cells; especially suitable for suspension cell culture. |
| Recommended Buffer System | Bicarbonate buffer system (Sodium Bicarbonate, 2000 mg/L). |
| Application Notes / Precautions | Store at 2-8 °C protected from light; uses a bicarbonate buffer system and requires a CO2 atmosphere to maintain physiological pH. |
For research use only, not for clinical use.
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