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| Product Name | Gelatin, Type A |
| Catalog No. | RESK-0478 |
| Description | Gelatin, also known as animal glue, is a heterogeneous mixture of high-molecular-weight water-soluble proteins found in collagen. It can be extracted from boiled animal tissues such as skin, tendons, ligaments, and bones. Depending on the extraction method, gelatin is classified as Type A (acid process) or Type B (alkaline process). The main biological applications of gelatin include: 1) coating cell culture plates/dishes to improve cell adhesion; 2) addition to PCR systems to improve the stability of Taq polymerase; 3) use as a blocking solution in Western blotting, ELISA, and immunohistochemistry; 4) in bacteriology and cell culture, inhibiting crystallization and serving as an important component of media for species classification and identification; 5) as a biocompatible polymer, serving as a carrier matrix for the release of bioactive macromolecules or as a regenerative scaffold in tissue regeneration engineering. Gelatin is also used in the pharmaceutical industry as a suspending agent, encapsulating agent, and tablet binder, and in veterinary medicine as a plasma expander and hemostatic agent. This product is Type A gelatin obtained by acid hydrolysis and purification of collagen from porcine skin, and is suitable for cell culture. A coating concentration of 0.1-0.2 mg/cm2 or 5-10 uL/cm2 is recommended; the optimal working concentration should be determined according to cell type, application, and research purpose. |
| Intended Use | Cell culture grade gelatin for coating cell culture plates/dishes to improve cell adhesion; also used as a blocking reagent in Western blotting, ELISA, and immunohistochemistry, as a PCR additive to stabilize Taq polymerase, and as a biocompatible carrier matrix. |
| Principle / Technology | Type A gelatin is a water-soluble protein mixture obtained by acid hydrolysis of collagen from porcine skin. Its Bloom number (240-270 Bloom) reflects the water-binding capacity, which is proportional to molecular weight. |
| Performance Range / Specifications | CAS No.: 9000-70-8 Grade: cell culture grade Appearance: off-white to yellowish-brown powder Bloom number: 240-270 Bloom Solubility: soluble in water; swells to 5-10 times its original weight in water below 35-40 °C to form a colloid; more readily soluble in hot water than in cold water; soluble in glycerol and acetic acid; almost insoluble in most organic reagents such as ethanol and chloroform |
| Reaction Conditions / Protocol | 1. Dissolving gelatin: weigh the appropriate amount of gelatin powder according to the desired concentration and add it to a container with double-distilled water or PBS buffer. a) After soaking for 10 min, heat in a hot water bath with stirring until completely dissolved (temperature should not exceed 70 °C); b) alternatively, after soaking for 10 min, heat gently (temperature should not exceed 70 °C) with constant stirring until completely dissolved. 2. Sterilization: a) autoclaving: dispense the prepared gelatin solution (commonly 0.1%-2.0%) into bottles and autoclave at 121 °C for 20 min at 15 psi; b) filter sterilization: low-concentration gelatin solutions (e.g., 0.1% or 0.2%) can be sterilized by filtration through a 0.2 um membrane after dissolving. Note: prepare extra filters, as gelatin is difficult to filter. 3. Storage of solutions: gelatin solutions are relatively stable; prepared solutions stored at 2-8 °C may retain performance for a long period. Note: at elevated temperatures, the strength and viscosity of gelatin decrease markedly and performance is compromised. 4. Coating procedure (the specific concentration and amount depend on cell type and experimental purpose): a) Prepare a 2% (w/v) gelatin solution with tissue culture grade water (e.g., double-distilled water). b) Autoclave at 121 °C for 20 min at 15 psi. c) Apply to the surface of culture dishes or flasks at 5-10 uL/cm2 (or 0.1-0.2 mg/cm2), fully covering the surface to be coated. d) Place in a 37 °C incubator for at least 2 h to dry the gelatin surface. Remove and aspirate excess gelatin. It is recommended to rinse once with culture medium and dry briefly before use. |
| Storage Conditions | Store at room temperature. Prepared gelatin solutions should be stored at 2-8 °C. |
| Shelf Life | 2 years |
| Package Specifications | 100 g |
| Product Form | Off-white to yellowish-brown powder |
| Quality Control | Cell culture grade; Bloom number 240-270 Bloom. |
| Source / Origin | Porcine skin; collagen purified by acid hydrolysis (Type A gelatin). |
| Regulatory / Compliance | For research use only. |
| Recommended Buffer System | Dissolve in double-distilled water or PBS buffer. |
| Application Notes / Precautions | Do not exceed 70 °C when dissolving gelatin. Gelatin is difficult to filter, so prepare extra filters for filter sterilization. At elevated temperatures, the strength and viscosity of gelatin decrease markedly and performance is compromised. The optimal coating concentration should be determined according to cell type, application, and research purpose. |
For research use only, not for clinical use.
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