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| Product Name | Human Liver Cancer Organoid Culture Kit |
| Catalog No. | RESK-0703 |
| Description | The Human Liver Cancer Organoid Culture Kit consists of two components: an organoid culture basement membrane matrix (phenol red-free, LDEV-free, EHS tumor-derived) and a human liver cancer organoid growth medium Plus. The matrix is a sterile preparation extracted from Engelbreth-Holm-Swarm (EHS) mouse tumors, mainly containing laminin, type IV collagen, heparan sulfate proteoglycan (HSPG), entactin (nidogen), and growth factors such as TGF-β and EGF. At room temperature it polymerizes to form a biologically active three-dimensional matrix that mimics the structure and function of the in vivo basement membrane. It has been validated for organoid culture and provides the 3D environmental support required for organoid construction. The human liver cancer organoid growth medium Plus is a serum-free formulation containing proprietary components and abundant cytokines. It enables liver cancer cells to grow rapidly and form organoids in an efficient and stable manner while maintaining good cellular characteristics and viability. This kit is used to efficiently establish and long-term culture human liver cancer organoids derived from cells or tissues, providing support for physiological function research of liver cancer, liver cancer disease model construction, and precision medicine support. |
| Intended Use | For the efficient establishment and long-term culture of human liver cancer organoids derived from cells or tissues, supporting physiological function research, disease model construction, and precision medicine. For research use only. |
| Principle / Technology | Organoid culture (three-dimensional cell culture). The basement membrane matrix polymerizes at room temperature to form a biologically active 3D matrix that mimics the structure and function of the in vivo basement membrane, providing the three-dimensional environment required for organoid construction. The serum-free growth medium supplies proprietary components and cytokines that promote rapid liver cancer cell growth and stable organoid formation. |
| Sample Type | Cells and tissues of human origin |
| Performance Range / Specifications | Complete and convenient combination: the basement membrane matrix and growth medium components are supplied together as an integrated kit. Efficient and stable: the serum-free formulation, combined with the validated basement membrane matrix, promotes rapid and stable organoid formation. |
| Components / Formulation | Component 1: Organoid culture basement membrane matrix (phenol red-free, LDEV-free, EHS tumor-derived). Main constituents: laminin, type IV collagen, heparan sulfate proteoglycan (HSPG), entactin (nidogen), and growth factors such as TGF-β and EGF. Sterile preparation. Component 2: Human Liver Cancer Organoid Growth Medium Plus — serum-free formulation containing proprietary components and abundant cytokines that promote rapid liver cancer cell growth and organoid formation. |
| Storage Conditions | Part I: store at 2-8 °C protected from light; valid for 12 months. Part II: store at -25 °C to -15 °C; valid for 12 months. Part III: store at -25 °C to -15 °C; valid for 24 months. |
| Shelf Life | Part I: 12 months at 2-8 °C protected from light. Part II: 12 months at -25 °C to -15 °C. Part III: 24 months at -25 °C to -15 °C. |
| Package Specifications | 1 KIT |
| Product Form | Liquid kit components (basement membrane matrix and serum-free growth medium), supplied frozen or refrigerated as specified. |
| Quality Control | Sterile preparation. Basement membrane matrix: phenol red-free, LDEV-free. Growth medium: serum-free formulation. Validated for organoid culture. |
| Source / Origin | Basement membrane matrix: extracted from Engelbreth-Holm-Swarm (EHS) mouse tumors. Growth medium and organoids: human (Homo sapiens) applications. |
| Regulatory / Compliance | For research use only. |
| Application Notes / Precautions | Store each component under its specified conditions. For research use only. |
For research use only, not for clinical use.
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