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| Product Name | trans-Zeatin |
| Catalog No. | RESK-0592 |
| Description | Zeatin is a natural plant cytokinin (cytokinins, CKs) present in higher plants. It was first discovered and isolated from young corn cobs, and was later also found in coconut milk together with its derivatives. As a plant growth regulator, it promotes lateral bud growth, stimulates cell differentiation (apical dominance), promotes callus formation and seed germination, prevents leaf senescence, reverses toxin damage to buds, and suppresses excessive root formation. High concentrations of zeatin can also induce adventitious bud differentiation. Zeatin exists as cis and trans isomers. The trans isomer has strong biological activity, whereas the cis isomer is relatively weak. trans-Zeatin exerts its function by binding with high affinity to receptors, including the Arabidopsis histidine kinases (AHKs); in particular, binding of the AHK3 receptor to trans-zeatin plays an important role in regulating plant metabolism. This product is high-purity trans-zeatin with a content of >97% and is suitable for plant tissue culture. |
| Intended Use | Used as a high-activity cytokinin plant growth regulator in plant tissue culture. |
| Principle / Technology | trans-Zeatin is the highly active trans isomer of the natural cytokinin zeatin. It activates its function by binding with high affinity to cytokinin receptors, including the Arabidopsis histidine kinases (AHKs); binding of trans-zeatin to the AHK3 receptor plays an important role in regulating plant metabolism. |
| Detection Method | Purity determined by HPLC. |
| Performance Range / Specifications | Synonyms: 6-(trans-4-Hydroxy-3-methyl-2-butenylamino)purine; N6-(trans-4-Hydroxy-isopentenyl)adenine CAS No.: 1637-39-4 Molecular formula: C10H13N5O Molecular weight: 219.24 Appearance: White crystals or powder Melting point: 207-208 °C Solubility: Soluble in 1 M acetic acid Purity (HPLC): >97% |
| Reaction Conditions / Protocol | 1) Stock solution preparation: Upon receipt, centrifuge the vial at low speed. Fully dissolve the powder in 5 mL of 1 M NaOH (or 1 M acetic acid). Then add double-distilled water with stirring to prepare a stock solution of 1 mg/mL or higher, ensuring thorough mixing. Aliquot the stock solution and store frozen; avoid repeated freeze-thaw cycles. 2) Recommended application concentrations: a) Promotion of callus shoot induction (must be used in combination with an auxin): 1 mg/L; b) Promotion of fruit set: zeatin 100 mg/L + GA3 500 mg/L + NAA 20 mg/L, sprayed onto fruits 10, 25, and 40 days after flowering; c) Delaying leaf yellowing of vegetables: 20 mg/L spray. |
| Storage Conditions | Store at -25 to -15 °C. |
| Package Specifications | 10 mg, 50 mg, 100 mg, 250 mg, 1 g |
| Product Form | White crystals or powder |
| Purity | >97% (HPLC) |
| Concentration | Stock solution: 1 mg/mL or higher; typical working concentrations range from 1 mg/L to 100 mg/L depending on the application. |
| Molecular Weight | 219.24 |
| Source / Origin | The trans isomer of zeatin, a natural cytokinin of higher plants first discovered and isolated from young corn cobs and also found in coconut milk. |
| Expiration Date / Stability | Aliquot the stock solution and store frozen; avoid repeated freeze-thaw cycles. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Used in combination with auxins for callus shoot induction; can be applied together with GA3 and NAA to promote fruit set. |
| Recommended Buffer System | Dissolve the powder in 1 M NaOH or 1 M acetic acid, then dilute with double-distilled water to the desired concentration. |
| Application Notes / Precautions | Centrifuge the vial at low speed upon receipt before opening. Aliquot stock solutions and avoid repeated freeze-thaw cycles. For callus shoot induction, trans-zeatin must be used in combination with an auxin. |
For research use only, not for clinical use.
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