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Fetal Bovine Serum (Premium Pro Grade)

Cat.No: RESK-0234 Datasheet

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Product Name Fetal Bovine Serum (Premium Pro Grade)
Catalog No. RESK-0234
Description Fetal Bovine Serum (Premium Pro Grade) is a high-quality serum supplement that is triple-filtered through 100 nm filters and screened for mycoplasma and viruses. After rigorous quality testing, the serum is filled on a fully automated production line under standardized manufacturing conditions to ensure consistent quality. It is rich in nutrients required for cell growth and is suitable for the culture of various primary cells, stem cells, immune cells and most routine cell lines. Advantages: Broad cell compatibility: suitable for routine culture of primary cells, stem cells and immune cells, including difficult-to-culture primary cells (chondrocytes, glial cells, cardiomyocytes, smooth muscle cells, epithelial cells, etc.), stem cells (mesenchymal stem cells, MSCs), difficult-to-culture immune cells (H9, NK and T cells, etc.), and various routine and hard-to-culture tumor cells. Excellent lot-to-lot stability: virtually no performance differences between batches. Rapid cell proliferation: performance comparable to high-quality reference sera, with stable cell status, short doubling times and rapid proliferation. Superior product quality: low antibody levels and high growth factor content.
Intended Use Used as a growth supplement in cell culture media for the in vitro culture of primary cells, stem cells (e.g., MSCs), immune cells (e.g., NK and T cells) and routine or difficult-to-culture cell lines. For research use.
Principle / Technology Triple 100 nm filtration sterilization combined with mycoplasma and virus screening, followed by fully automated aseptic filling under standardized production conditions.
Performance Range / Specifications Endotoxin: ≤5 EU/mL
Hemoglobin: ≤200 mg/L
Mycoplasma: negative
Sterilization: triple 100 nm filtration
Reaction Conditions / Protocol Thawing (choose one of two methods):
1) Remove the serum from -20°C storage and place it overnight in a 2-8°C refrigerator. Then transfer the serum to a 37°C water bath, swirling the bottle occasionally to mix. Do not leave the thawed serum at 37°C for an extended period.
2) Remove the serum directly from -20°C and place it in a 37°C water bath, shaking the bottle continuously to accelerate thawing and mixing.
Components / Formulation Sterile-filtered fetal bovine serum rich in nutrients and growth factors required for cell growth, with low antibody levels and high growth factor content.
Storage Conditions Store at -20°C to -10°C for up to 5 years.
Once thawed, keep the serum at 2-8°C and use within 6 weeks. For long-term storage after thawing, aliquot and refreeze; avoid repeated freeze-thaw cycles.
Shelf Life 5 years when stored at -20°C to -10°C.
Package Specifications 500 mL
50 mL
10 mL
Product Form Frozen liquid serum
Quality Control Each lot is tested for endotoxin (≤5 EU/mL), hemoglobin (≤200 mg/L), mycoplasma (negative) and viruses (screened), and is sterilized by triple 100 nm filtration. Cell culture performance is verified across multiple cell types (e.g., HaCat, 293T, RAW264.7, THP-1, Huh7 and HepG2 cells) for growth status and viability.
Source / Origin Fetal bovine blood.
Shipping Conditions Shipped on dry ice.
Expiration Date / Stability Stable for 5 years at -20°C to -10°C; after thawing, stable for up to 6 weeks at 2-8°C.
Compatibility Compatible with a wide range of cell types, including human tumor cell lines (e.g., HeLa, HepG2, Huh-7, A549, MCF7, PANC-1, SH-SY5Y, OVCAR-3, K562, Jurkat), primary cells (chondrocytes, glial cells, cardiomyocytes, smooth muscle cells, epithelial cells), stem cells (human/mouse/rat BMSCs, ADSCs, neural stem cells), immune cells (H9, NK and T cells, macrophages such as RAW264.7 and THP-1), and rodent/primate cell lines (CHO, Vero, 3T3-L1, L6, NRK-52E).
Application Notes / Precautions If the bottle is not shaken during thawing and the temperature exceeds 40°C, material deposited at the bottom of the bottle may undergo protein denaturation and form precipitates. Avoid prolonged exposure to high temperatures and repeated freeze-thaw cycles.
Batch-to-Batch Consistency Virtually no lot-to-lot differences; product performance is highly stable across different batches.

For research use only, not for clinical use.

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