- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Human Fibronectin Coating Solution, 1 mg/mL |
| Catalog No. | ATR-CCR-0018 |
| Description | Purified human plasma fibronectin at 1 mg/mL in a sterile solution for coating tissue culture surfaces to promote attachment and spreading of anchorage-dependent cells, particularly primary cells and stem cells. |
| Intended Use | Coating tissue culture plastic and glass surfaces to enhance cell adhesion; improving attachment and spreading of primary cells, endothelial cells, and mesenchymal stem cells; supporting cell migration studies. |
| Principle / Technology | Fibronectin contains RGD peptide motifs recognized by integrin receptors on the cell surface; binding triggers focal adhesion formation and actin cytoskeleton reorganization, promoting cell spreading and survival signaling. |
| Detection Method | Cell attachment rate measurement, cell spreading area quantification by microscopy |
| Sample Type | Primary human cells, endothelial cells (HUVEC), mesenchymal stem cells, epithelial cells, fibroblasts |
| Performance Range / Specifications | Optimal coating concentration 1–5 µg/cm²; >90% cell attachment with 30–60 minute incubation |
| Sensitivity / LOD | N/A |
| Specificity | N/A |
| Reaction Conditions / Protocol | Dilute in sterile PBS to desired coating concentration; add to tissue culture surface; incubate 1–2 hours at 37°C or overnight at 4°C; aspirate remaining solution; rinse with PBS; use immediately. |
| Components / Formulation | Human plasma fibronectin 1 mg/mL in PBS with 10% glycerol, sterile-filtered |
| Storage Conditions | -20°C; avoid repeated freeze-thaw cycles |
| Shelf Life | 12 months at -20°C |
| Package Specifications | 1 mg (1 mL), 5 mg (5 mL) |
| Product Form | Liquid, sterile |
| Quality Control | Purity by SDS-PAGE >95%, biological activity by cell attachment assay, sterility, endotoxin |
| Key Features | Human plasma derived; RGD-mediated cell adhesion; sterile; validated for primary cell attachment; compatible with most cell types |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
