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| Product Name | XhoI Rapid Restriction Endonuclease (20 U/uL) |
| Catalog No. | IVDRM-0484 |
| Description | Cleaving 5 prime C^TCGAG 3 prime, this rapid recombinant endonuclease completes precise DNA cutting in 5 to 15 minutes and suits fast digestion of plasmid DNA, PCR products and genomic DNA. It is supplied at 20 U/uL, has an optimal reaction temperature of 37°C in the shared rapid series buffer and is heat inactivated at 80°C for 20 minutes. CpG methylation fully overlapping the sequence restricts cutting, and PaeR7I, TliI, BssHI, Sfr274I, SlaI and StrI are isoschizomers sharing the specificity. |
| Intended Use | DNA fragment cleavage and rapid digestion of plasmid DNA, PCR products and genomic DNA |
| Principle / Technology | Site specific cleavage at 5 prime C^TCGAG 3 prime within 5 to 15 minutes at 37°C |
| Sample Type | Plasmid DNA; PCR products; genomic DNA |
| Specificity | CpG methylation fully overlapping the sequence restricts cleavage; isoschizomers PaeR7I, TliI, BssHI, Sfr274I, SlaI and StrI |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer at 37°C for 5 to 15 minutes; heat inactivate at 80°C for 20 minutes |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 500 T |
| Key Features | Single shared buffer across the rapid series; broad isoschizomer family |
| Concentration | 20 U/uL |
| Readouts / Downstream Assays | Agarose gel profile of digested DNA |
For in vitro diagnostic use.
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