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| Product Name | XbaI Rapid Restriction Endonuclease, High Concentration (100 U/uL) |
| Catalog No. | IVDRM-0485 |
| Description | A hyper-concentrated format of the rapid XbaI endonuclease for vector construction and routine DNA digestion, this preparation recognises 5 prime T^CTAGA 3 prime and completes cutting within 5 to 15 minutes. It is supplied at 100 U/uL in the shared rapid series buffer, has an optimal reaction temperature of 37°C and is heat inactivated at 80°C for 20 minutes. Dam methylation overlapping the sequence can block cutting, and it ships on ice packs. |
| Intended Use | Vector construction and DNA digestion of plasmid DNA, PCR products and genomic DNA |
| Principle / Technology | Site specific cleavage at 5 prime T^CTAGA 3 prime within 5 to 15 minutes at 37°C |
| Sample Type | Plasmid DNA; PCR products; genomic DNA |
| Specificity | Dam methylation overlapping the sequence can block cleavage |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer at 37°C for 5 to 15 minutes; heat inactivate at 80°C for 20 minutes |
| Storage Conditions | -20°C |
| Shelf Life | 2 years |
| Package Specifications | 10,000 U |
| Key Features | High concentration format suited to large scale digestion; single shared buffer across the rapid series |
| Concentration | 100 U/uL |
| Shipping Conditions | Ice pack shipping |
| Readouts / Downstream Assays | Agarose gel analysis of digested DNA |
For in vitro diagnostic use.
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