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| Product Name | Universal Cell Freezing Medium, Sterile |
| Catalog No. | RERE-0195 |
| Description | Sterile, ready-to-use freezing medium for preservation of cultured cells, formulated from serum, basal culture medium and a cryoprotective agent. The pre-mixed format removes the need to prepare fresh freeze mix per experiment and keeps composition consistent across batches. Recommended practice is controlled cooling at roughly 1°C per minute; where a programmed freezer is unavailable, a stepwise scheme of 20 minutes at 4°C, 30 minutes at -20°C and overnight at -70°C before transfer to liquid nitrogen works acceptably. Avoid repeated freeze-thaw of the stock. Unopened bottles keep for 24 months at -20°C. |
| Intended Use | Cryopreservation of adherent and suspension cultured cells in liquid nitrogen storage |
| Principle / Technology | Serum-based carrier with cryoprotectant limits intracellular ice formation and osmotic injury during controlled cooling |
| Specificity | Sterile solution; serum, basal medium and cryoprotectant composition; 24 month shelf life |
| Reaction Conditions / Protocol | Resuspend cells in chilled freezing medium at the working density, aliquot into cryovials and cool at about 1°C per minute, or follow the 4°C, -20°C, -70°C stepwise scheme, then transfer to liquid nitrogen |
| Storage Conditions | -20°C |
| Package Specifications | 50 mL | 100 mL |
| Key Features | Pre-mixed and sterile; supports the standard 1°C per minute cooling profile; 24 month shelf life |
For research use only, not for clinical use.
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