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| Product Name | UniPhi29 DNA Polymerase, 42°C Reaction Mutant (10 U/uL) |
| Catalog No. | IVDRM-0574 |
| Description | An engineered mutant of wild type phi29 DNA polymerase from a Bacillus subtilis phage, UniPhi29 sustains DNA synthesis continuously at 42°C, a reaction characteristic that markedly improves assay performance. The enzyme keeps the strong 3 prime to 5 prime exonuclease proofreading activity of the parent enzyme for high fidelity synthesis, and because of that activity primers need a 3 prime end phosphorothioate modification to reduce primer cutting by the exonuclease. Applications include whole genome amplification, multiple displacement amplification and rolling circle amplification with improved yield and efficiency and reduced non-specific amplification, plus NGS library workflows where coverage uniformity rises, the required sequencing depth falls and gap regions shrink, and single cell sequencing with better data quality and completeness. |
| Intended Use | WGA, MDA and RCA with improved uniformity for NGS and single cell sequencing |
| Principle / Technology | Processive strand-displacing synthesis at 42°C with strong 3 prime to 5 prime proofreading |
| Sample Type | Genomic DNA and circular templates for uniform amplification |
| Specificity | Continuous synthesis at 42°C; primers require 3 prime phosphorothioate modification |
| Reaction Conditions / Protocol | Incubate at 42°C with thiophosphate-modified primers for uniform amplification |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 250 U; 1000 U; 10000 U |
| Key Features | Elevated temperature reaction improves coverage uniformity and lowers required sequencing depth |
| Concentration | 10 U/uL |
| Readouts / Downstream Assays | Sequencing coverage uniformity and amplification yield |
For in vitro diagnostic use.
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