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| Product Name | Ultra Reverse Transcriptase, RNase H-Free (200 U/µL) |
| Catalog No. | IVDRM-0316 |
| Description | An engineered reverse transcriptase derived from an RNase H-deficient M-MLV backbone. Removal of RNase H activity greatly improves full-length cDNA synthesis up to 20 kb, and thermal stability carries reactions to 65 °C so templates with complex secondary structures reverse-transcribe efficiently. Processivity, synthesis rate and inhibitor tolerance are all raised, addressing the low efficiency caused by inhibitors in difficult samples, and detection reaches 1 pg of total RNA in five minutes. |
| Intended Use | Full-length cDNA library construction, end-point PCR, RT-PCR and real-time RT-PCR, RNA-seq library preparation and single-cell sequencing. |
| Detection Method | RNA templates including structured and inhibitor-containing samples |
| Sample Type | Total RNA, mRNA |
| Performance Range / Specifications | Activity 200 U/µL; synthesised cDNA up to 20 kb; reaction temperature up to 65 °C; reverse transcription in 5 minutes; sensitivity to 1 pg total RNA |
| Specificity | Lacks RNase H activity; carries non-template nucleotide addition activity |
| Storage Conditions | -20 °C |
| Shelf Life | 1 year |
| Package Specifications | 2000 U, 10000 U, 200000 U |
| Quality Control | No RNase H activity; no detectable nicking enzyme, exonuclease or RNase residue; host genomic DNA residue at trace levels |
| Concentration | 200 U/µL |
| Shipping Conditions | Dry ice |
| Readouts / Downstream Assays | Real-time quantitative PCR, agarose gel electrophoresis |
For in vitro diagnostic use.
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