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| Product Name | Ultra-Low Residual Murine RNase Inhibitor (40 U/uL) |
| Catalog No. | IVDRM-0582 |
| Description | A recombinant murine RNase inhibitor expressed and purified in soluble form from E. coli, which inactivates ribonucleases by binding them non-covalently into an inactive complex and broadly inhibits RNase A, RNase B and RNase C. Because it lacks the two oxidation-sensitive cysteines of the human protein it has higher antioxidant activity and suits high DTT sensitivity assays such as qPCR, and RT-PCR and RT-qPCR testing confirms compatibility with reverse transcriptases and various DNA polymerases. This ultra-low residual format holds E. coli host DNA below 0.1 copies per 100 U, stays active from 25 to 60°C across pH 5.0 to 9.0, and is aimed at applications with strict background requirements. |
| Intended Use | RNase protection in reverse transcription and qPCR assays and as a molecular diagnostic raw material |
| Principle / Technology | Non-covalent complex formation with RNase A, B and C that inactivates the enzymes |
| Sample Type | RNA-containing reaction systems in pathogen detection and molecular diagnostics |
| Specificity | Host DNA residue below 0.1 copies per 100 U; active from 25 to 60°C across pH 5.0 to 9.0 |
| Reaction Conditions / Protocol | Add to the reaction system at the working concentration to protect RNA templates and products |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 2 KU; 10 KU; 20 KU; 100 KU; 1000 KU; 20000 KU |
| Key Features | Ultra-low host DNA residue; high antioxidant activity for oxidation-sensitive workflows |
| Concentration | 40 U/uL |
| Readouts / Downstream Assays | RT-qPCR performance with protected RNA templates |
For in vitro diagnostic use.
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