- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Tsp45I Rapid Restriction Endonuclease (10 U/uL) |
| Catalog No. | IVDRM-0477 |
| Description | Cutting 5 prime ^GTSAC 3 prime, this rapid recombinant endonuclease digests plasmid DNA, PCR products and genomic DNA within 5 to 15 minutes and performs best at a reaction temperature of 65°C, which is well matched to thermophilic workflows. It is supplied at 10 U/uL in the unified rapid series buffer. EcoBI methylation overlapping the site restricts cutting whereas Dam, Dcm, CpG and EcoKI methylation have no effect, and NmuCI together with TseFI are isoschizomers. The enzyme cannot be heat inactivated, so remove it afterwards by phenol chloroform extraction or column purification. |
| Intended Use | Rapid digestion of plasmid DNA, PCR products and genomic DNA at elevated temperature |
| Principle / Technology | Site specific cleavage at 5 prime ^GTSAC 3 prime within 5 to 15 minutes at 65°C |
| Sample Type | Plasmid DNA; PCR products; genomic DNA |
| Specificity | EcoBI methylation restricts cleavage while Dam, Dcm, CpG and EcoKI methylation have no effect; isoschizomers NmuCI and TseFI |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer at 65°C for 5 to 15 minutes; not heat inactivatable, remove enzyme by phenol chloroform extraction or column purification |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 25 T |
| Key Features | Elevated 65°C reaction temperature suited to thermophilic templates; single shared buffer across the rapid series |
| Concentration | 10 U/uL |
| Readouts / Downstream Assays | Agarose gel analysis of digested DNA |
For in vitro diagnostic use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
