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| Product Name | TseI (ApeKI) Rapid Restriction Endonuclease (10 U/uL) |
| Catalog No. | IVDRM-0478 |
| Description | Recognising the degenerate five base site 5 prime G^CWGC 3 prime, this rapid recombinant endonuclease processes plasmid DNA, PCR products and genomic DNA within 5 to 15 minutes. It is supplied at 10 U/uL, works best at 65°C and is heat inactivated at 80°C for 20 minutes. CpG methylation overlapping the site restricts cutting, and ApeKI is an isoschizomer with identical specificity. |
| Intended Use | Rapid digestion of plasmid DNA, PCR products and genomic DNA for molecular cloning |
| Principle / Technology | Site specific cleavage at 5 prime G^CWGC 3 prime within 5 to 15 minutes at 65°C |
| Sample Type | Plasmid DNA; PCR products; genomic DNA |
| Specificity | CpG methylation overlapping the site restricts cleavage; isoschizomer ApeKI |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer at 65°C for 5 to 15 minutes; heat inactivate at 80°C for 20 minutes |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 25 T |
| Key Features | Degenerate recognition site; single shared buffer across the rapid series |
| Concentration | 10 U/uL |
| Readouts / Downstream Assays | Agarose gel profile of digested DNA |
For in vitro diagnostic use.
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