Trypsin Solution, 2.5%, Sterile (EDTA-Free)
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Trypsin Solution, 2.5%, Sterile (EDTA-Free)

Cat.No: RERE-0198 Datasheet

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Product Name Trypsin Solution, 2.5%, Sterile (EDTA-Free)
Catalog No. RERE-0198
Description Concentrated sterile trypsin solution at 2.5 percent without EDTA, filter-sterilised and intended either for direct use in demanding dissociation tasks, such as primary tissue disruption, or for dilution into working-strength solutions prepared in-house. The tenfold concentration relative to the standard 0.25 percent grade gives laboratories latitude over strength and buffer composition. Handle aseptically and avoid repeated freeze-thaw. Unopened aliquots keep for 12 months at -20°C.
Intended Use Concentrated stock for in-house dilution to working strength and for dissociation tasks needing higher proteolytic activity, including tissue disruption
Principle / Technology Trypsin cleaves cell-surface adhesion proteins; the concentrated format permits flexible dilution to the strength a workflow needs
Specificity Sterile 2.5 percent trypsin, filter-sterilised; no EDTA; 12 month shelf life
Reaction Conditions / Protocol Dilute to the working strength with a balanced salt or buffer base before routine passaging; when used directly, monitor detachment closely and quench promptly with serum-containing medium
Storage Conditions -20°C
Package Specifications 100 mL
Key Features Tenfold concentrated relative to the standard grade; EDTA-free; filter-sterilised; 12 month shelf life

For research use only, not for clinical use.

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