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| Product Name | Trypsin Solution, 0.25%, with Phenol Red, Sterile (EDTA-Free) |
| Catalog No. | RERE-0219 |
| Description | Sterile trypsin solution at the conventional 0.25 percent strength without EDTA, formulated with phenol red for visual pH tracking through the detachment step. The standard strength covers routine passaging and subculturing of adherent lines, and the indicator colour helps confirm the solution stayed within range during warming and use. Handle aseptically and avoid repeated freeze-thaw. Unopened aliquots keep for 12 months at -20°C. |
| Intended Use | Routine dissociation and passaging of adherent cultured cells with visual pH indication |
| Principle / Technology | Standard trypsin strength cleaves adhesion proteins for routine detachment while phenol red tracks pH status visually |
| Specificity | Sterile 0.25 percent trypsin, filter-sterilised; with phenol red; no EDTA; 12 month shelf life |
| Reaction Conditions / Protocol | Warm to room temperature or 37°C before use, rinse the monolayer with a balanced salt wash, add enough solution to cover and incubate briefly until cells detach, then quench with serum-containing medium |
| Storage Conditions | -20°C |
| Package Specifications | 100 mL |
| Key Features | Conventional 0.25 percent strength with phenol red indicator; EDTA-free; 12 month shelf life |
For research use only, not for clinical use.
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