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| Product Name | Trypsin Solution (0.25%), with Phenol Red |
| Catalog No. | RESK-0243 |
| Description | This product is a trypsin solution formulated in Hanks' Balanced Salt Solution (HBSS), containing 2.5 g/L trypsin (1:250) with phenol red as a pH indicator, and free of EDTA, CaCl2, MgCl2·6H2O, and MgSO4·7H2O. It is supplied as a 1x working solution and can be used directly for the dissociation of tissues and adherent cell monolayers. The product is sterile filtered and has been tested for mycoplasma. |
| Intended Use | Dissociation of tissues and adherent cell monolayers, cell disaggregation from adherent surfaces, and tissue dispersion during primary cell passaging in routine cell culture. |
| Principle / Technology | Trypsin is a serine protease commonly found in the digestive system of vertebrates and is secreted by the pancreas as the inactive precursor trypsinogen. It hydrolyzes peptide chains primarily at the carboxyl side of lysine or arginine residues (except when the adjacent residue is proline). During cell dissociation, trypsin degrades extracellular adhesion proteins, releasing adherent cells from the culture surface and dispersing cell aggregates. |
| Sample Type | Adherent cultured cells, cell monolayers, and tissues |
| Performance Range / Specifications | Sterility: sterile filtered Chelator: no EDTA Indicator: phenol red Classification: animal-derived Appearance: liquid pH range: 7.2-8.0 Osmolality: 270-320 mOsm/kg |
| Reaction Conditions / Protocol | Supplied as a 1x working solution; apply directly to tissues or cell monolayers for dissociation. |
| Components / Formulation | 2.5 g/L trypsin (1:250) in Hanks' Balanced Salt Solution (HBSS) With phenol red (pH indicator) No EDTA Free of CaCl2, MgCl2·6H2O, and MgSO4·7H2O Supplied as a 1x ready-to-use working solution |
| Storage Conditions | Store at -25 to -15 °C. Aliquoting before freezing at -25 to -15 °C is recommended; avoid repeated freeze-thaw cycles. |
| Shelf Life | 2 years |
| Package Specifications | 100 mL |
| Product Form | Liquid, 1x ready-to-use working solution |
| Quality Control | Sterile filtered; tested for mycoplasma contamination. |
| Concentration | 2.5 g/L trypsin (1:250), 0.25% |
| Source / Origin | Porcine pancreas (animal-derived) |
| pH Range / Optimal pH | 7.2-8.0 |
| Shipping Conditions | Shipped with ice packs |
| Expiration Date / Stability | Stable for 2 years when stored at -25 to -15 °C; digestive activity decreases over prolonged storage. Aliquot and freeze; avoid long-term storage at 4 °C. |
| Recommended Buffer System | Hanks' Balanced Salt Solution (HBSS) |
| Application Notes / Precautions | Color variation may occur: CO2 exchange during cold-chain transport can shift the solution pH from 7.2-8.0 (light red) to around 6.8 (light yellow); this pH-driven color change is a common phenomenon and the discolored solution still dissociates cells effectively and can be used with confidence. Aliquot before freezing and avoid repeated freeze-thaw cycles. Because this formulation contains no EDTA, residual trypsin solution does not need to be removed after digestion. |
| Batch-to-Batch Consistency | Osmolality controlled at 270-320 mOsm/kg; pH controlled at 7.2-8.0. |
| Recognition Site | Cleaves peptide bonds at the carboxyl-terminal side of lysine and arginine residues (except when the adjacent residue is proline). |
For research use only, not for clinical use.
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