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| Product Name | Trypsin Solution, 0.25%, Sterile (EDTA-Free) |
| Catalog No. | RERE-0197 |
| Description | Sterile trypsin solution at 0.25 percent without EDTA, supplied filter-sterilised for routine dissociation of adherent cultured cells and selected tissue samples. The EDTA-free composition suits lines whose downstream workflows disfavour chelator carry-over, and pairs naturally with a balanced salt base containing calcium and magnesium. Handle aseptically and avoid repeated freeze-thaw, which degrades activity. Unopened aliquots keep for 12 months at -20°C. |
| Intended Use | Routine dissociation and passaging of adherent cultured cells and preparation of dissociated tissue samples |
| Principle / Technology | Trypsin cleaves cell-surface adhesion proteins to release cells from the substrate and from each other |
| Specificity | Sterile 0.25 percent trypsin, filter-sterilised; no EDTA; 12 month shelf life |
| Reaction Conditions / Protocol | Warm to room temperature or 37°C before use, rinse the monolayer with a balanced salt wash, add enough solution to cover and incubate briefly until cells detach, then quench with serum-containing medium |
| Storage Conditions | -20°C |
| Package Specifications | 100 mL | 500 mL | 10 x 100 mL |
| Key Features | Standard 0.25 percent strength; EDTA-free; filter-sterilised; 12 month shelf life |
For research use only, not for clinical use.
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