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| Product Name | Trypsin Solution (0.25%), No EDTA, Phenol Red-Free |
| Catalog No. | RESK-0241 |
| Description | This product is a trypsin solution formulated in Hanks' Balanced Salt Solution (HBSS), containing 2.5 g/L trypsin (1:250) and free of EDTA, CaCl2, MgCl2·6H2O, MgSO4·7H2O, and phenol red. It is supplied as a 1x working solution and can be used directly for the dissociation of tissues and adherent cell monolayers. The product has been tested for mycoplasma. The phenol red-free formulation is especially suited for flow cytometry (avoiding phenol red interference with fluorescence signals), hormone-related research (e.g., estrogen-sensitive studies on breast cancer or endometrial cells), and serum-free or specialized culture systems (phenol red may disturb sodium-potassium balance or medium composition). |
| Intended Use | Direct dissociation of tissues and adherent cell monolayers in routine cell culture. The phenol red-free formulation is recommended for flow cytometry, hormone-related research, and serum-free or specialized culture systems. |
| Principle / Technology | Trypsin is a serine protease commonly found in the digestive system of vertebrates and is secreted by the pancreas as the inactive precursor trypsinogen. It hydrolyzes peptide chains primarily at the carboxyl side of lysine or arginine residues (except when the adjacent residue is proline). During cell dissociation, trypsin degrades extracellular adhesion proteins, releasing adherent cells from the culture surface and dispersing cell aggregates. |
| Components / Formulation | 2.5 g/L trypsin (1:250) in Hanks' Balanced Salt Solution (HBSS) No EDTA No phenol red Free of CaCl2, MgCl2·6H2O, and MgSO4·7H2O Supplied as a 1x ready-to-use working solution |
| Storage Conditions | Store at -25 to -15 °C. Aliquoting before freezing at -25 to -15 °C is recommended; avoid repeated freeze-thaw cycles. |
| Shelf Life | 2 years |
| Package Specifications | 100 mL |
| Product Form | Liquid, 1x ready-to-use working solution |
| Quality Control | Tested for mycoplasma contamination. |
| Concentration | 2.5 g/L trypsin (1:250), 0.25% |
| Source / Origin | Porcine pancreas (animal-derived) |
| Shipping Conditions | Shipped with ice packs |
| Expiration Date / Stability | Stable for 2 years when stored at -25 to -15 °C; digestive activity decreases over prolonged storage. Aliquot and freeze; avoid long-term storage at 4 °C. |
| Recommended Buffer System | Hanks' Balanced Salt Solution (HBSS) |
| Application Notes / Precautions | Aliquot before freezing and avoid repeated freeze-thaw cycles. Because this formulation contains no EDTA, residual trypsin solution does not need to be removed after digestion. |
| Recognition Site | Cleaves peptide bonds at the carboxyl-terminal side of lysine and arginine residues (except when the adjacent residue is proline). |
For research use only, not for clinical use.
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